The impact of post-treatment of digestate prior to its recirculation to the digester has been evaluated with industrial-scale ultrasonication and electrokinetic treatment units. Residual methane yields of untreated digestate samples from four biogas plants varied between 99 and 134 ml/g of volatile solids (after 97 days of digestion). At the tested conditions (1.90–7.60 kWh/t for ultrasonication and 4.37–6.06 kWh/t for electrokinetic disintegration), no significant increase in methane yields were obtained. Increases in maximum methane production rates of up to 42% were observed in samples from one of the biogas plants, resulting in net energy gains between 1.06 and 7.04 kWh/t (after 26 and 66 days of digestion). Both treatments significantly reduced digestate viscosities, especially of highly viscous samples. Lower viscosities could reduce the energy demand for pumping and stirring in the digester, enabling the potential for increased organic loading and increased methane production.