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Droplet Microfluidics Platform for Highly Sensitive and Quantitative Detection of Malaria-Causing Plasmodium Parasites Based on Enzyme Activity Measurement

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  • Sissel Juul, Department of Biomedical Engineering, Duke University, USA
  • Christine Juul Fælled Nielsen
  • Rodrigo Labouriau
  • Amit Roy, Danmark
  • Cinzia Tesauro
  • Pia Wrensted Jensen
  • ,
  • Charlotte Harmsen, Danmark
  • Emil Laust Kristoffersen
  • Ya-Ling Chiu, Department of Biomedical Engineering, Duke University, USA
  • Rikke Frøhlich Hougaard
  • Paola Fiorani, Institute of Translational Pharmacoloy, National Research Council, Italien
  • Janet Cox-Singh, School of Medicine, University of St. Andrews, Storbritannien
  • David Paul Tordrup, Danmark
  • Jørn Erland Koch, Danmark
  • Anne-Losi Bienvenu, Malaria Research Unit, Frankrig
  • Alessandro Desideri, Department of Biology, University of Rome, Italien
  • Stephane Picot, Malaria Research Unit, Frankrig
  • Eskild Petersen
  • Kam W Leong, Department of Biomedical Engineering, Duke University, USA
  • Yi-Ping Ho, Danmark
  • Magnus Stougaard
  • Birgitta R Knudsen
We present an attractive new system for the specific and sensitive detection of the malaria-causing Plasmodium parasites. The system relies on isothermal conversion of single DNA cleavage–ligation events catalyzed specifically by the Plasmodium enzyme topoisomerase I to micrometer-sized products detectable at the single-molecule level. Combined with a droplet microfluidics lab-on-a-chip platform, this design allowed for sensitive, specific, and quantitative detection of all human-malaria-causing Plasmodium species in single drops of unprocessed blood with a detection limit of less than one parasite/μL. Moreover, the setup allowed for detection of Plasmodium parasites in noninvasive saliva samples from infected patients. During recent years malaria transmission has declined worldwide, and with this the number of patients with low-parasite density has increased. Consequently, the need for accurate detection of even a few parasites is becoming increasingly important for the continued combat against the disease. We believe that the presented droplet microfluidics platform, which has a high potential for adaptation to point-of-care setups suitable for low-resource settings, may contribute significantly to meet this demand. Moreover, potential future adaptation of the presented setup for the detection of other microorganisms may form the basis for the development of a more generic platform for diagnosis, fresh water or food quality control, or other purposes within applied or basic science.
OriginalsprogEngelsk
TidsskriftA C S Nano
Vol/bind6
Nummer12
Sider (fra-til)10676-10683
Antal sider8
ISSN1936-0851
DOI
StatusUdgivet - dec. 2012

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