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DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection

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  • V. Ferrara, Università di Catania
  • ,
  • A. Ottaviani, Universita degli Studi di Roma Tor Vergata
  • ,
  • F. Cavaleri, Universita degli Studi di Palermo
  • ,
  • G. Arrabito, Universita degli Studi di Palermo
  • ,
  • P. Cancemi, Universita degli Studi di Palermo
  • ,
  • Y. P. Ho, Chinese University Hong Kong
  • ,
  • B. R. Knudsen
  • M. S. Hede
  • C. Pellerito, Universita degli Studi di Palermo
  • ,
  • A. Desideri, Universita degli Studi di Roma Tor Vergata
  • ,
  • S. Feo, Universita degli Studi di Palermo
  • ,
  • Giovanni Marletta, Università di Catania
  • ,
  • B. Pignataro, Universita degli Studi di Palermo

Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.

OriginalsprogEngelsk
TitelSensors
RedaktørerBruno Andò, Francesco Baldini, Pietro Siciliano, Marco Rossi, Lorenzo Scalise, Corrado Di Natale, Vittorio Ferrari, Valeria Militello, Giorgia Miolo, Vincenzo Marletta, Giovanna Marrazza
Antal sider8
ForlagSpringer
Udgivelsesår18 jan. 2019
Sider309-316
ISBN (trykt)9783030043230
ISBN (Elektronisk)978-3-030-04324-7
DOI
StatusUdgivet - 18 jan. 2019
Begivenhed4th National Conference on Sensors, 2018 - Catania, Italien
Varighed: 21 feb. 201823 feb. 2018

Konference

Konference4th National Conference on Sensors, 2018
LandItalien
ByCatania
Periode21/02/201823/02/2018
SerietitelLecture Notes in Electrical Engineering
Vol/bind539
ISSN1876-1100

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