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DNA Topoisomerases Are Required for Preinitiation Complex Assembly during GAL Gene Activation

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DNA Topoisomerases Are Required for Preinitiation Complex Assembly during GAL Gene Activation. / Roedgaard, Morten; Fredsoe, Jacob; Pedersen, Jakob Madsen; Bjergbaek, Lotte; Andersen, Anni Hangaard.

I: PLOS ONE, Bind 10, Nr. 7, e0132739, 14.07.2015, s. 1-15.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{1ad6e4c0a7e04f97b213d6070b9c0ef7,
title = "DNA Topoisomerases Are Required for Preinitiation Complex Assembly during GAL Gene Activation",
abstract = "To investigate the importance of topoisomerases for transcription of the galactose induced genes, we have studied the expression of GAL1, GAL2, GAL7 and GAL10 in Saccharomyces cerevisiae cells deficient for topoisomerases I and II. We find that topoisomerases are required for transcriptional activation of the GAL genes, but are dispensable for ongoing transcription, eliminating a role of the enzymes in transcriptional elongation. Furthermore, we demonstrate that promoter chromatin remodeling of the GAL genes is unaffected in the topoisomerase deficient strain. However, the cells fail to successfully recruit RNA polymerase II due to an inability of the TATA-binding protein (TBP) to bind to the TATA box in these promoters. We therefore argue that topoisomerases are required for accurate assembly of the preinitiation complex at the promoters of the GAL genes.",
author = "Morten Roedgaard and Jacob Fredsoe and Pedersen, {Jakob Madsen} and Lotte Bjergbaek and Andersen, {Anni Hangaard}",
year = "2015",
month = jul,
day = "14",
doi = "10.1371/journal.pone.0132739",
language = "English",
volume = "10",
pages = "1--15",
journal = "P L o S One",
issn = "1932-6203",
publisher = "public library of science",
number = "7",

}

RIS

TY - JOUR

T1 - DNA Topoisomerases Are Required for Preinitiation Complex Assembly during GAL Gene Activation

AU - Roedgaard, Morten

AU - Fredsoe, Jacob

AU - Pedersen, Jakob Madsen

AU - Bjergbaek, Lotte

AU - Andersen, Anni Hangaard

PY - 2015/7/14

Y1 - 2015/7/14

N2 - To investigate the importance of topoisomerases for transcription of the galactose induced genes, we have studied the expression of GAL1, GAL2, GAL7 and GAL10 in Saccharomyces cerevisiae cells deficient for topoisomerases I and II. We find that topoisomerases are required for transcriptional activation of the GAL genes, but are dispensable for ongoing transcription, eliminating a role of the enzymes in transcriptional elongation. Furthermore, we demonstrate that promoter chromatin remodeling of the GAL genes is unaffected in the topoisomerase deficient strain. However, the cells fail to successfully recruit RNA polymerase II due to an inability of the TATA-binding protein (TBP) to bind to the TATA box in these promoters. We therefore argue that topoisomerases are required for accurate assembly of the preinitiation complex at the promoters of the GAL genes.

AB - To investigate the importance of topoisomerases for transcription of the galactose induced genes, we have studied the expression of GAL1, GAL2, GAL7 and GAL10 in Saccharomyces cerevisiae cells deficient for topoisomerases I and II. We find that topoisomerases are required for transcriptional activation of the GAL genes, but are dispensable for ongoing transcription, eliminating a role of the enzymes in transcriptional elongation. Furthermore, we demonstrate that promoter chromatin remodeling of the GAL genes is unaffected in the topoisomerase deficient strain. However, the cells fail to successfully recruit RNA polymerase II due to an inability of the TATA-binding protein (TBP) to bind to the TATA box in these promoters. We therefore argue that topoisomerases are required for accurate assembly of the preinitiation complex at the promoters of the GAL genes.

U2 - 10.1371/journal.pone.0132739

DO - 10.1371/journal.pone.0132739

M3 - Journal article

C2 - 26173127

VL - 10

SP - 1

EP - 15

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 7

M1 - e0132739

ER -