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DNA REPAIR. Mus81 and converging forks limit the mutagenicity of replication fork breakage

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DNA REPAIR. Mus81 and converging forks limit the mutagenicity of replication fork breakage. / Mayle, Ryan; Campbell, Ian M; Beck, Christine R; Yu, Yang; Wilson, Marenda; Shaw, Chad A; Bjergbaek, Lotte; Lupski, James R; Ira, Grzegorz.

I: Science, Bind 349, Nr. 6249, 14.08.2015, s. 742-747.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Mayle, R, Campbell, IM, Beck, CR, Yu, Y, Wilson, M, Shaw, CA, Bjergbaek, L, Lupski, JR & Ira, G 2015, 'DNA REPAIR. Mus81 and converging forks limit the mutagenicity of replication fork breakage', Science, bind 349, nr. 6249, s. 742-747. https://doi.org/10.1126/science.aaa8391

APA

Mayle, R., Campbell, I. M., Beck, C. R., Yu, Y., Wilson, M., Shaw, C. A., Bjergbaek, L., Lupski, J. R., & Ira, G. (2015). DNA REPAIR. Mus81 and converging forks limit the mutagenicity of replication fork breakage. Science, 349(6249), 742-747. https://doi.org/10.1126/science.aaa8391

CBE

Mayle R, Campbell IM, Beck CR, Yu Y, Wilson M, Shaw CA, Bjergbaek L, Lupski JR, Ira G. 2015. DNA REPAIR. Mus81 and converging forks limit the mutagenicity of replication fork breakage. Science. 349(6249):742-747. https://doi.org/10.1126/science.aaa8391

MLA

Vancouver

Mayle R, Campbell IM, Beck CR, Yu Y, Wilson M, Shaw CA o.a. DNA REPAIR. Mus81 and converging forks limit the mutagenicity of replication fork breakage. Science. 2015 aug 14;349(6249):742-747. https://doi.org/10.1126/science.aaa8391

Author

Mayle, Ryan ; Campbell, Ian M ; Beck, Christine R ; Yu, Yang ; Wilson, Marenda ; Shaw, Chad A ; Bjergbaek, Lotte ; Lupski, James R ; Ira, Grzegorz. / DNA REPAIR. Mus81 and converging forks limit the mutagenicity of replication fork breakage. I: Science. 2015 ; Bind 349, Nr. 6249. s. 742-747.

Bibtex

@article{09532f3831bd41639419c9c68f0e0af0,
title = "DNA REPAIR. Mus81 and converging forks limit the mutagenicity of replication fork breakage",
abstract = "Most spontaneous DNA double-strand breaks (DSBs) result from replication-fork breakage. Break-induced replication (BIR), a genome rearrangement-prone repair mechanism that requires the Pol32/POLD3 subunit of eukaryotic DNA Polδ, was proposed to repair broken forks, but how genome destabilization is avoided was unknown. We show that broken fork repair initially uses error-prone Pol32-dependent synthesis, but that mutagenic synthesis is limited to within a few kilobases from the break by Mus81 endonuclease and a converging fork. Mus81 suppresses template switches between both homologous sequences and diverged human Alu repetitive elements, highlighting its importance for stability of highly repetitive genomes. We propose that lack of a timely converging fork or Mus81 may propel genome instability observed in cancer.",
keywords = "Alu Elements, Base Sequence, DNA Breaks, Double-Stranded, DNA Repair, DNA Replication, DNA-Binding Proteins, DNA-Directed DNA Polymerase, Endonucleases, Genomic Instability, Humans, Molecular Sequence Data, Neoplasms, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins",
author = "Ryan Mayle and Campbell, {Ian M} and Beck, {Christine R} and Yang Yu and Marenda Wilson and Shaw, {Chad A} and Lotte Bjergbaek and Lupski, {James R} and Grzegorz Ira",
note = "Copyright {\textcopyright} 2015, American Association for the Advancement of Science.",
year = "2015",
month = aug,
day = "14",
doi = "10.1126/science.aaa8391",
language = "English",
volume = "349",
pages = "742--747",
journal = "Science",
issn = "0036-8075",
publisher = "AMER ASSOC ADVANCEMENT SCIENCE",
number = "6249",

}

RIS

TY - JOUR

T1 - DNA REPAIR. Mus81 and converging forks limit the mutagenicity of replication fork breakage

AU - Mayle, Ryan

AU - Campbell, Ian M

AU - Beck, Christine R

AU - Yu, Yang

AU - Wilson, Marenda

AU - Shaw, Chad A

AU - Bjergbaek, Lotte

AU - Lupski, James R

AU - Ira, Grzegorz

N1 - Copyright © 2015, American Association for the Advancement of Science.

PY - 2015/8/14

Y1 - 2015/8/14

N2 - Most spontaneous DNA double-strand breaks (DSBs) result from replication-fork breakage. Break-induced replication (BIR), a genome rearrangement-prone repair mechanism that requires the Pol32/POLD3 subunit of eukaryotic DNA Polδ, was proposed to repair broken forks, but how genome destabilization is avoided was unknown. We show that broken fork repair initially uses error-prone Pol32-dependent synthesis, but that mutagenic synthesis is limited to within a few kilobases from the break by Mus81 endonuclease and a converging fork. Mus81 suppresses template switches between both homologous sequences and diverged human Alu repetitive elements, highlighting its importance for stability of highly repetitive genomes. We propose that lack of a timely converging fork or Mus81 may propel genome instability observed in cancer.

AB - Most spontaneous DNA double-strand breaks (DSBs) result from replication-fork breakage. Break-induced replication (BIR), a genome rearrangement-prone repair mechanism that requires the Pol32/POLD3 subunit of eukaryotic DNA Polδ, was proposed to repair broken forks, but how genome destabilization is avoided was unknown. We show that broken fork repair initially uses error-prone Pol32-dependent synthesis, but that mutagenic synthesis is limited to within a few kilobases from the break by Mus81 endonuclease and a converging fork. Mus81 suppresses template switches between both homologous sequences and diverged human Alu repetitive elements, highlighting its importance for stability of highly repetitive genomes. We propose that lack of a timely converging fork or Mus81 may propel genome instability observed in cancer.

KW - Alu Elements

KW - Base Sequence

KW - DNA Breaks, Double-Stranded

KW - DNA Repair

KW - DNA Replication

KW - DNA-Binding Proteins

KW - DNA-Directed DNA Polymerase

KW - Endonucleases

KW - Genomic Instability

KW - Humans

KW - Molecular Sequence Data

KW - Neoplasms

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins

U2 - 10.1126/science.aaa8391

DO - 10.1126/science.aaa8391

M3 - Journal article

C2 - 26273056

VL - 349

SP - 742

EP - 747

JO - Science

JF - Science

SN - 0036-8075

IS - 6249

ER -