Disulphide-mediated site-directed modification of proteins

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

DOI

  • Thorbjørn Nielsen
  • Anders Märcher
  • Zuzana Drobňáková, APIGENEX S.r.o.
  • ,
  • Michal Hučko, APIGENEX S.r.o.
  • ,
  • Milan Štengl, APIGENEX S.r.o.
  • ,
  • Vojtěch Balšánek, APIGENEX S.r.o.
  • ,
  • Charlotte Wiberg, Novo Nordisk A/S
  • ,
  • Per F. Nielsen, Novo Nordisk A/S
  • ,
  • Thomas E. Nielsen, Novo Nordisk A/S
  • ,
  • Kurt V. Gothelf
  • Emiliano Cló, Novo Nordisk A/S

Methods for chemical modification of native proteins in a controlled fashion are in high demand. Here, a novel protocol that exploits bifunctional reagents for transient targeting of solvent exposed disulphides to direct the introduction of a single exogenous reactive thiol handle at a lysine side chain has been developed. The protocol has successfully been applied to functionalize six different Fabs and human growth hormone. This journal is

OriginalsprogEngelsk
TidsskriftOrganic and Biomolecular Chemistry
Vol/bind18
Nummer25
Sider (fra-til)4717-4722
Antal sider6
ISSN1477-0520
DOI
StatusUdgivet - jul. 2020

Se relationer på Aarhus Universitet Citationsformater

ID: 192495950