Direct gene transfer in the Gottingen minipig CNS using stereotaxic lentiviral microinjections

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  • Institut for Medicinsk Biokemi
  • Molekylær Genetik og Systembiologi
  • Kandidatuddannelse i Biomedicinsk Teknik
  • Anatomisk Institut
We aim to induce direct viral mediated gene transfer in the substantia nigra (SN) of the Gottingen minipig using MRI guided stereotaxic injections of lentiviral vectors encoding enhanced green fluorescent protein (EGFP). Nine female Gottingen minipigs were injected unilaterally into the SN with 6 per 2.5 microliters lentivirus capable of transducing cells and mediating expression of recombinant EGFP. The animals were euthanized after four (n=3) or twenty weeks (n=6). Fresh brain tissue from three animals was used for PCR. The remaining six brains were cryo- or paraffin-sectioned for fluorescence, Nissl-, and immunohistochemical EGFP visualization. EGFP was seen in nigral neurons, axons, glial cells, endothelial cells, and in nigral fibers targeting the striatum. PCR-based detection confirmed the presence of the transgene in SN, whereas all other examined brain areas were negative, indicating that the immunohistochemically detected EGFP in the striatum derived from transfected nigral cells.
OriginalsprogEngelsk
TidsskriftActa Neurobiologiae Experimentalis
Vol/bind70
Nummer3
Sider (fra-til)308-315
Antal sider8
ISSN0065-1400
StatusUdgivet - 2010

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