Differential regulation of the Epr3 receptor coordinates membrane-restricted rhizobial colonization of root nodule primordia

TY - JOUR

T1 - Differential regulation of the Epr3 receptor coordinates membrane-restricted rhizobial colonization of root nodule primordia

AU - Kawaharada, Yasuyuki

AU - Nielsen, Mette W

AU - Kelly, Simon

AU - James, Euan K

AU - Andersen, Kasper Røjkjær

AU - Rasmussen, Sheena R

AU - Füchtbauer, Winnie

AU - Madsen, Lene H

AU - Heckmann, Anne B

AU - Radutoiu, Simona

AU - Stougaard, Jens

PY - 2017/2/23

Y1 - 2017/2/23

N2 - In Lotus japonicus, a LysM receptor kinase, EPR3, distinguishes compatible and incompatible rhizobial exopolysaccharides at the epidermis. However, the role of this recognition system in bacterial colonization of the root interior is unknown. Here we show that EPR3 advances the intracellular infection mechanism that mediates infection thread invasion of the root cortex and nodule primordia. At the cellular level, Epr3 expression delineates progression of infection threads into nodule primordia and cortical infection thread formation is impaired in epr3 mutants. Genetic dissection of this developmental coordination showed that Epr3 is integrated into the symbiosis signal transduction pathways. Further analysis showed differential expression of Epr3 in the epidermis and cortical primordia and identified key transcription factors controlling this tissue specificity. These results suggest that exopolysaccharide recognition is reiterated during the progressing infection and that EPR3 perception of compatible exopolysaccharide promotes an intracellular cortical infection mechanism maintaining bacteria enclosed in plant membranes.

AB - In Lotus japonicus, a LysM receptor kinase, EPR3, distinguishes compatible and incompatible rhizobial exopolysaccharides at the epidermis. However, the role of this recognition system in bacterial colonization of the root interior is unknown. Here we show that EPR3 advances the intracellular infection mechanism that mediates infection thread invasion of the root cortex and nodule primordia. At the cellular level, Epr3 expression delineates progression of infection threads into nodule primordia and cortical infection thread formation is impaired in epr3 mutants. Genetic dissection of this developmental coordination showed that Epr3 is integrated into the symbiosis signal transduction pathways. Further analysis showed differential expression of Epr3 in the epidermis and cortical primordia and identified key transcription factors controlling this tissue specificity. These results suggest that exopolysaccharide recognition is reiterated during the progressing infection and that EPR3 perception of compatible exopolysaccharide promotes an intracellular cortical infection mechanism maintaining bacteria enclosed in plant membranes.

KW - Journal Article

U2 - 10.1038/ncomms14534

DO - 10.1038/ncomms14534

M3 - Journal article

C2 - 28230048

SN - 2041-1723

VL - 8

JO - Nature Communications

JF - Nature Communications

M1 - 14534

ER -