Differential Incorporation of Bacteria, Organic Matter, and Inorganic Ions Into Lake Ice During Ice Formation

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

DOI

  • Pamela A. Santibáñez, Montana State Univ, Montana State University, Montana State University System, Montana State University Bozeman, Dept Ecol, Instituto Antártico Chileno
  • ,
  • Alexander B. Michaud
  • Trista J. Vick-Majors, Montana State Univ, Montana State University, Montana State University System, Montana State University Bozeman, Dept Ecol, College of Forestry and Conservation, The University of Montana, Missoula, Montana, USA
  • ,
  • Juliana D'Andrilli, Montana State Univ, Montana State University, Montana State University System, Montana State University Bozeman, Dept Ecol
  • ,
  • Amy Chiuchiolo, Montana State Univ, Montana State University, Montana State University System, Montana State University Bozeman, Dept Ecol
  • ,
  • Kevin P. Hand, Jet Propulsion Laboratory/California Institute of Technology
  • ,
  • John C. Priscu, Montana State Univ, Montana State University, Montana State University System, Montana State University Bozeman, Dept Ecol

The segregation of bacteria, inorganic solutes, and total organic carbon between liquid water and ice during winter ice formation on lakes can significantly influence the concentration and survival of microorganisms in icy systems and their roles in biogeochemical processes. Our study quantifies the distributions of bacteria and solutes between liquid and solid water phases during progressive freezing. We simulated lake ice formation in mesocosm experiments using water from perennially (Antarctica) and seasonally (Alaska and Montana, United States) ice-covered lakes. We then computed concentration factors and effective segregation coefficients, which are parameters describing the incorporation of bacteria and solutes into ice. Experimental results revealed that, contrary to major ions, bacteria were readily incorporated into ice and did not concentrate in the liquid phase. The organic matter incorporated into the ice was labile, amino acid-like material, differing from the humic-like compounds that remained in the liquid phase. Results from a control mesocosm experiment (dead bacterial cells) indicated that viability of bacterial cells did not influence the incorporation of free bacterial cells into ice, but did have a role in the formation and incorporation of bacterial aggregates. Together, these findings demonstrate that bacteria, unlike other solutes, were preferentially incorporated into lake ice during our freezing experiments, a process controlled mainly by the initial solute concentration of the liquid water source, regardless of cell viability.

OriginalsprogEngelsk
TidsskriftJournal of Geophysical Research: Biogeosciences
Vol/bind124
Nummer3
Sider (fra-til)585-600
Antal sider16
ISSN2169-8953
DOI
StatusUdgivet - mar. 2019

Se relationer på Aarhus Universitet Citationsformater

ID: 160593876