Differential Cell Lysis Among Periodontal Strains of JP2 and Non-JP2 Genotype of Aggregatibacter actinomycetemcomitans Serotype B Is Not Reflected in Dissimilar Expression and Production of Leukotoxin

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DOI

  • Anne Birkeholm Jensen
  • Marianne Lund
  • ,
  • Niels Nørskov-Lauritsen
  • Anders Johansson, Section of Molecular Periodontology, Department of Odontology, Faculty of Medicine, Umeå University, 901 87 Umeå, Sweden. anders.p.johansson@umu.se.
  • ,
  • Rolf Claesson, Division of Oral Microbiology, Department of Odontology, Faculty of Medicine, Umeå University, 901 87 Umeå, Sweden. rolf.claesson@umu.se.
  • ,
  • Jesper Reinholdt
  • Dorte Haubek

Leukotoxic potential of Aggregatibacter actinomycetemcomitans strains has been studied by the use of several methods, and results differ depending on the methods used. The aim of the present study was to perform a comprehensive examination of the leukotoxic potential of a collection of A. actinomycetemcomitans strains by use of three quantitative methods, Western blotting, ELISA, and mRNA expression assay and compare these results with previous data obtained by a cell lysis assay. A higher leukotoxic potential among JP2 genotype strains compared to non-JP2 genotype strains of A. actinomycetemcomitans was found by Western blotting, ELISA and mRNA expression assay. Leukotoxicity as determined by cell lysis assay showed a variation among strains examined, not only depending on being part of JP2 genotype vs. non-JP2 genotype group of A. actinomycetemcomitans. The leukotoxicity of A. actinomycetemcomitans strains as determined by cell lysis assay did not correspond to the leukotoxic potential of A. actinomycetemcomitans strains as determined by three quantitative methods. A comparison of the results obtained by ELISA and mRNA expression assay showed a reasonable correlation between these two methods. It seems important to use more than one method to assess the LtxA-related virulence capacity of A. actinomycetemcomitans in order to obtain comprehensive understanding of the leukotoxic potential of A. actinomycetemcomitans strains.

OriginalsprogEngelsk
Artikelnummer211
TidsskriftPathogens
Vol/bind8
Nummer4
ISSN2076-0817
DOI
StatusUdgivet - 30 okt. 2019

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