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Dietary proanthocyanidins promote localized antioxidant responses in porcine pulmonary and gastrointestinal tissues during Ascaris suum-induced type 2 inflammation

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Dietary proanthocyanidins promote localized antioxidant responses in porcine pulmonary and gastrointestinal tissues during Ascaris suum-induced type 2 inflammation. / Andersen-Civil, Audrey Inge Schytz; Myhill, Laura J.; Büdeyri Gökgöz, Nilay et al.
I: FASEB Journal, Bind 36, Nr. 4, e22256, 04.2022.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Andersen-Civil, AIS, Myhill, LJ, Büdeyri Gökgöz, N, Engström, MT, Mejer, H, Zhu, L, Zeller, WE, Salminen, JP, Krych, L, Lauridsen, C, Nielsen, DS, Thamsborg, SM & Williams, AR 2022, 'Dietary proanthocyanidins promote localized antioxidant responses in porcine pulmonary and gastrointestinal tissues during Ascaris suum-induced type 2 inflammation', FASEB Journal, bind 36, nr. 4, e22256. https://doi.org/10.1096/fj.202101603RR

APA

Andersen-Civil, A. I. S., Myhill, L. J., Büdeyri Gökgöz, N., Engström, M. T., Mejer, H., Zhu, L., Zeller, W. E., Salminen, J. P., Krych, L., Lauridsen, C., Nielsen, D. S., Thamsborg, S. M., & Williams, A. R. (2022). Dietary proanthocyanidins promote localized antioxidant responses in porcine pulmonary and gastrointestinal tissues during Ascaris suum-induced type 2 inflammation. FASEB Journal, 36(4), artikel e22256. https://doi.org/10.1096/fj.202101603RR

CBE

Andersen-Civil AIS, Myhill LJ, Büdeyri Gökgöz N, Engström MT, Mejer H, Zhu L, Zeller WE, Salminen JP, Krych L, Lauridsen C, et al. 2022. Dietary proanthocyanidins promote localized antioxidant responses in porcine pulmonary and gastrointestinal tissues during Ascaris suum-induced type 2 inflammation. FASEB Journal. 36(4):Article e22256. https://doi.org/10.1096/fj.202101603RR

MLA

Vancouver

Andersen-Civil AIS, Myhill LJ, Büdeyri Gökgöz N, Engström MT, Mejer H, Zhu L et al. Dietary proanthocyanidins promote localized antioxidant responses in porcine pulmonary and gastrointestinal tissues during Ascaris suum-induced type 2 inflammation. FASEB Journal. 2022 apr.;36(4):e22256. doi: 10.1096/fj.202101603RR

Author

Andersen-Civil, Audrey Inge Schytz ; Myhill, Laura J. ; Büdeyri Gökgöz, Nilay et al. / Dietary proanthocyanidins promote localized antioxidant responses in porcine pulmonary and gastrointestinal tissues during Ascaris suum-induced type 2 inflammation. I: FASEB Journal. 2022 ; Bind 36, Nr. 4.

Bibtex

@article{28b7d200a79945bc84c17b435f3f6ebe,
title = "Dietary proanthocyanidins promote localized antioxidant responses in porcine pulmonary and gastrointestinal tissues during Ascaris suum-induced type 2 inflammation",
abstract = "Proanthocyanidins (PAC) are dietary polyphenols with putative anti-inflammatory and immunomodulatory effects. However, whether dietary PAC can regulate type-2 immune function and inflammation at mucosal surfaces remains unclear. Here, we investigated if diets supplemented with purified PAC modulated pulmonary and intestinal mucosal immune responses during infection with the helminth parasite Ascaris suum in pigs. A. suum infection induced a type-2 biased immune response in lung and intestinal tissues, characterized by pulmonary granulocytosis, increased Th2/Th1 T cell ratios in tracheal-bronchial lymph nodes, intestinal eosinophilia, and modulation of genes involved in mucosal barrier function and immunity. Whilst PAC had only minor effects on pulmonary immune responses, RNA-sequencing of intestinal tissues revealed that dietary PAC significantly enhanced transcriptional responses related to immune function and antioxidant responses in the gut of both na{\"i}ve and A. suum-infected animals. A. suum infection and dietary PAC induced distinct changes in gut microbiota composition, primarily in the jejunum and colon, respectively. Notably, PAC consumption substantially increased the abundance of Limosilactobacillus reuteri. In vitro experiments with porcine macrophages and intestinal epithelial cells supported a role for both PAC polymers and PAC-derived microbial metabolites in regulating oxidative stress responses in host tissues. Thus, dietary PAC may have distinct beneficial effects on intestinal health during infection with mucosal pathogens, while having a limited activity to modulate naturally-induced type-2 pulmonary inflammation. Our results shed further light on the mechanisms underlying the health-promoting properties of PAC-rich foods, and may aid in the design of novel dietary supplements to regulate mucosal inflammatory responses in the gastrointestinal tract.",
keywords = "Ascaris suum, gut microbiota, helminth, pig, polyphenols, proanthocyandins",
author = "Andersen-Civil, {Audrey Inge Schytz} and Myhill, {Laura J.} and {B{\"u}deyri G{\"o}kg{\"o}z}, Nilay and Engstr{\"o}m, {Marica T.} and Helena Mejer and Ling Zhu and Zeller, {Wayne E.} and Salminen, {Juha Pekka} and Lukasz Krych and Charlotte Lauridsen and Nielsen, {Dennis S.} and Thamsborg, {Stig M.} and Williams, {Andrew R.}",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.",
year = "2022",
month = apr,
doi = "10.1096/fj.202101603RR",
language = "English",
volume = "36",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FEDERATION AMER SOC EXP BIOL",
number = "4",

}

RIS

TY - JOUR

T1 - Dietary proanthocyanidins promote localized antioxidant responses in porcine pulmonary and gastrointestinal tissues during Ascaris suum-induced type 2 inflammation

AU - Andersen-Civil, Audrey Inge Schytz

AU - Myhill, Laura J.

AU - Büdeyri Gökgöz, Nilay

AU - Engström, Marica T.

AU - Mejer, Helena

AU - Zhu, Ling

AU - Zeller, Wayne E.

AU - Salminen, Juha Pekka

AU - Krych, Lukasz

AU - Lauridsen, Charlotte

AU - Nielsen, Dennis S.

AU - Thamsborg, Stig M.

AU - Williams, Andrew R.

N1 - Publisher Copyright: © 2022 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.

PY - 2022/4

Y1 - 2022/4

N2 - Proanthocyanidins (PAC) are dietary polyphenols with putative anti-inflammatory and immunomodulatory effects. However, whether dietary PAC can regulate type-2 immune function and inflammation at mucosal surfaces remains unclear. Here, we investigated if diets supplemented with purified PAC modulated pulmonary and intestinal mucosal immune responses during infection with the helminth parasite Ascaris suum in pigs. A. suum infection induced a type-2 biased immune response in lung and intestinal tissues, characterized by pulmonary granulocytosis, increased Th2/Th1 T cell ratios in tracheal-bronchial lymph nodes, intestinal eosinophilia, and modulation of genes involved in mucosal barrier function and immunity. Whilst PAC had only minor effects on pulmonary immune responses, RNA-sequencing of intestinal tissues revealed that dietary PAC significantly enhanced transcriptional responses related to immune function and antioxidant responses in the gut of both naïve and A. suum-infected animals. A. suum infection and dietary PAC induced distinct changes in gut microbiota composition, primarily in the jejunum and colon, respectively. Notably, PAC consumption substantially increased the abundance of Limosilactobacillus reuteri. In vitro experiments with porcine macrophages and intestinal epithelial cells supported a role for both PAC polymers and PAC-derived microbial metabolites in regulating oxidative stress responses in host tissues. Thus, dietary PAC may have distinct beneficial effects on intestinal health during infection with mucosal pathogens, while having a limited activity to modulate naturally-induced type-2 pulmonary inflammation. Our results shed further light on the mechanisms underlying the health-promoting properties of PAC-rich foods, and may aid in the design of novel dietary supplements to regulate mucosal inflammatory responses in the gastrointestinal tract.

AB - Proanthocyanidins (PAC) are dietary polyphenols with putative anti-inflammatory and immunomodulatory effects. However, whether dietary PAC can regulate type-2 immune function and inflammation at mucosal surfaces remains unclear. Here, we investigated if diets supplemented with purified PAC modulated pulmonary and intestinal mucosal immune responses during infection with the helminth parasite Ascaris suum in pigs. A. suum infection induced a type-2 biased immune response in lung and intestinal tissues, characterized by pulmonary granulocytosis, increased Th2/Th1 T cell ratios in tracheal-bronchial lymph nodes, intestinal eosinophilia, and modulation of genes involved in mucosal barrier function and immunity. Whilst PAC had only minor effects on pulmonary immune responses, RNA-sequencing of intestinal tissues revealed that dietary PAC significantly enhanced transcriptional responses related to immune function and antioxidant responses in the gut of both naïve and A. suum-infected animals. A. suum infection and dietary PAC induced distinct changes in gut microbiota composition, primarily in the jejunum and colon, respectively. Notably, PAC consumption substantially increased the abundance of Limosilactobacillus reuteri. In vitro experiments with porcine macrophages and intestinal epithelial cells supported a role for both PAC polymers and PAC-derived microbial metabolites in regulating oxidative stress responses in host tissues. Thus, dietary PAC may have distinct beneficial effects on intestinal health during infection with mucosal pathogens, while having a limited activity to modulate naturally-induced type-2 pulmonary inflammation. Our results shed further light on the mechanisms underlying the health-promoting properties of PAC-rich foods, and may aid in the design of novel dietary supplements to regulate mucosal inflammatory responses in the gastrointestinal tract.

KW - Ascaris suum

KW - gut microbiota

KW - helminth

KW - pig

KW - polyphenols

KW - proanthocyandins

UR - http://www.scopus.com/inward/record.url?scp=85127240565&partnerID=8YFLogxK

U2 - 10.1096/fj.202101603RR

DO - 10.1096/fj.202101603RR

M3 - Journal article

C2 - 35333423

AN - SCOPUS:85127240565

VL - 36

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 4

M1 - e22256

ER -