Dietary K+ acts as a genuine diuretic

Samuel L Svendsen, Simon Kornvig, Peder Berg, Iben S Jensen, Isabela B B A de Araujo, Casper K Larsen, Jens Leipziger, Mads V Sorensen*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Abstract

K+ balance in mammals relies on regulated renal K+ excretion matching unregulated fluctuating K+ intake. Upon a K+ rich meal, rapid and powerful K+ excretion is needed. Renal K+ secretion is stimulated by increased tubular flow. We speculated that high K+ intake acutely increases urinary flow to stimulate K+ excretion.

METHODS: Mice were K+ challenged through diets or gavage. Post K+ loading urinary output, osmolarity, [K+ ]u , [Na+ ]u , plasma osmolarity, [copeptin]p , [K+ ]p , and [Na+ ]p were measured. To locate the mechanism of K+ -induced diuresis in the glomerular/tubular system we measured creatinine excretion and assessed functional transport in isolated perfused TALs and CDs during an acute [K+ ]bl switch from 3.6 to 6.5 mM. Molecular adaptations of transport proteins involved in water reabsorption were investigated by immunoblotting.

RESULTS: 1) Mice switched from a 1% to 2% K+ diet increased diuresis within 12h and reciprocally reduced diuresis when switched from 1% to 0.01% K+ diet. 2) A single K+ gavage load, corresponding to 25-50% of daily K+ intake, induced 100% increase in diuresis within 30 minutes. This occurred despite augmented plasma osmolarity and AVP synthesis. 3) K+ gavage did not change GFR. 4) In isolated perfused TALs, shifting [K+ ]bl from 3.6 to 6.5 mM did not affect AVP-induced NaCl transport. 5) In sharp contrast, in isolated perfused CDs, shifting [K+ ]bl from 3.6 to 6.5 mM markedly reduced CD AVP-sensitivity, i.e. inhibited water absorption.

CONCLUSION: Dietary K+ loading induces a rapidly on-setting diuresis. The mechanism of K+ -induced diuresis involves desensitization of the CD to AVP.

OriginalsprogEngelsk
Artikelnummere13762
TidsskriftActa Physiologica
Vol/bind234
Nummer2
Antal sider18
ISSN1748-1708
DOI
StatusUdgivet - feb. 2022

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