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Development of a walleye cell line and use to study the effects of temperature on infection by viral haemorrhagic septicaemia virus group IVb

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

DOI

  • N T K Vo, University of Waterloo, Canada
  • A W Bender, University of Waterloo, Canada
  • L E J Lee, Faculty of Science, University of the Fraser Valley, Abbotsford, BC, Canada
  • J S Lumsden, University of Guelph, Canada
  • Niels Lorenzen, Danmark
  • B Dixon, University of Waterloo, Canada
  • N C Bols, University of Waterloo, Canada
A cell line, WE-cfin11f, with a fibroblast-like morphology was developed from a walleye caudal fin and used to study the intersection of thermobiology of walleye, Sander vitreus (Mitchill), with the thermal requirements for replication of viral haemorrhagic septicaemia virus (VHSV) IVb. WE-cfin11f proliferated from 10 to 32 °C and endured as a monolayer for at least a week at 1–34 °C. WE-cfin11f adopted an epithelial shape and did not proliferate at 4 °C. Adding VHSV IVb to cultures at 4 and 14 °C but not 26 °C led to cytopathic effects (CPE) and virus production. At 4 °C, virus production developed more slowly, but Western blotting showed more N protein accumulation. Infecting monolayer cultures at 4 °C for 7 days and then shifting them to 26 °C resulted in the monolayers being broken in small areas by CPE, but with time at 26 °C, the monolayers were restored. These results suggest that at 26 °C, the VHSV IVb life cycle stages responsible for CPE can be completed, but the production of virus and the initiation of infections cannot be accomplished.
OriginalsprogEngelsk
TidsskriftJournal of Fish Diseases Online
Vol/bind38
Nummer2
Sider (fra-til)121-136
ISSN1365-2761
DOI
StatusUdgivet - feb. 2015

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