Development and mapping of gene-tagged SNP markers in laccases of maize (Zea mays L.)

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  • J R Andersen, Danmark
  • T Asp
  • Y C Lu, College of Agricultural Resources and Environmental Sciences, Heilongjiang University, Kina
  • M Kloiber-Maitz, KWS Saat AG, Tyskland
  • M Ouzonova, KWS Saat AG, Tyskland
  • T Luebberstedt, Department of Agronomy, Iowa State University, USA
  • Institut for Genetik og Bioteknologi
  • Molekylær Genetik og Bioteknologi
Laccases, EC or p-diphenol : dioxygen oxidoreductases, have been proposed to be involved in the oxidative polymerization of monolignols into lignins in plants. While 17 laccases have been identified in Arabidopsis, only five (ZmLac1-5) have so far been identified in maize. By a bioinformatic approach, 14 putative laccases were identified in maize. One putative laccase was identical to ZmLac1, while five were highly homologous to either ZmLac4 or ZmLac5. Sequence alignment of allelic sequences enabled the development of TaqMan single nucleotide polymorphism (SNP) markers for nine putative laccases. Four of these gene-tagged SNP markers were validated in a doubled-haploid mapping population of 140 individuals, mapping these loci to chromosomes 1, 2, 3 and 7, respectively.
TidsskriftPlant Breeding
Sider (fra-til)423-425
Antal sider3
StatusUdgivet - 2009

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