Detoxification of toxin A and toxin B by copper ion-catalyzed oxidation in production of a toxoid-based vaccine against Clostridioides difficile

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  • Aria Aminzadeh, Statens Serum Institut, Københavns Universitet
  • ,
  • Manish Kumar Tiwari, Københavns Universitet
  • ,
  • Srwa Satar Mamah Mustapha, Statens Serum Institut
  • ,
  • Sandra Junquera Navarrete, Statens Serum Institut
  • ,
  • Anna Bielecka Henriksen, Statens Serum Institut
  • ,
  • Ian Max Møller
  • Karen Angeliki Krogfelt, Roskilde University
  • ,
  • Morten Jannik Bjerrum, Københavns Universitet
  • ,
  • René Jørgensen, Statens Serum Institut

Clostridioides difficile infections (CDI) has emerged worldwide as a serious antimicrobial-resistant healthcare-associated disease resulting in diarrhea and pseudomembranous colitis. The two cytotoxic proteins, toxin A (TcdA) and toxin B (TcdB) are the major virulence factor responsible for the disease symptoms. We examined time-dependent oxidative detoxification of TcdA and TcdB using different molar ratios of protein:Cu2+:H2O2. The metal-catalyzed oxidation (MCO) reaction in molar ratios of 1:60:1000 for protein:Cu2+:H2O2 at pH 4.5 resulted in a significant 6 log10 fold reduction in cytotoxicity after 120-min incubation at 37 °C. Circular dichroism revealed that MCO-detoxified TcdA and TcdB had secondary and tertiary structural folds similar to the native proteins. The conservation of immunogenic epitopes of both proteins was tested using monoclonal antibodies in an ELISA, comparing our MCO-detoxification approach to a conventional formaldehyde-detoxification method. The oxidative detoxification of TcdA and TcdB led to an average 2-fold reduction in antibody binding relative to native proteins, whereas formaldehyde cross-linking resulted in 3-fold and 5-fold reductions, respectively. Finally, we show that mice immunized with a vaccine consisting of MCO-detoxified TcdA and TcdB were fully protected against disease symptoms and death following a C. difficile infection and elicited substantial serum IgG responses against both TcdA and TcdB. The results of this study present copper ion-catalyzed oxidative detoxification of toxic proteins as a method highly suitable for the rapid production of safe, immunogenic and irreversible toxoid antigens for future vaccine development and may have the potential for replacing cross-linking reagents like formaldehyde.

OriginalsprogEngelsk
TidsskriftFree Radical Biology and Medicine
Vol/bind160
Sider (fra-til)433-446
Antal sider14
ISSN0891-5849
DOI
StatusUdgivet - nov. 2020

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