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Determination of recombination in Mycoplasma hominis

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Standard

Determination of recombination in Mycoplasma hominis. / Jacobsen, Iben Søgaard; Boesen, Thomas; Mygind, Tina; Melkova, Renata; Birkelund, Svend; Christiansen, Gunna; Schierup, Mikkel Heide.

I: Infect Genet Evol, Bind 1, 2002, s. 277-85.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Jacobsen, IS, Boesen, T, Mygind, T, Melkova, R, Birkelund, S, Christiansen, G & Schierup, MH 2002, 'Determination of recombination in Mycoplasma hominis', Infect Genet Evol, bind 1, s. 277-85.

APA

Jacobsen, I. S., Boesen, T., Mygind, T., Melkova, R., Birkelund, S., Christiansen, G., & Schierup, M. H. (2002). Determination of recombination in Mycoplasma hominis. Infect Genet Evol, 1, 277-85.

CBE

Jacobsen IS, Boesen T, Mygind T, Melkova R, Birkelund S, Christiansen G, Schierup MH. 2002. Determination of recombination in Mycoplasma hominis. Infect Genet Evol. 1:277-85.

MLA

Jacobsen, Iben Søgaard o.a.. "Determination of recombination in Mycoplasma hominis". Infect Genet Evol. 2002, 1. 277-85.

Vancouver

Jacobsen IS, Boesen T, Mygind T, Melkova R, Birkelund S, Christiansen G o.a. Determination of recombination in Mycoplasma hominis. Infect Genet Evol. 2002;1:277-85.

Author

Jacobsen, Iben Søgaard ; Boesen, Thomas ; Mygind, Tina ; Melkova, Renata ; Birkelund, Svend ; Christiansen, Gunna ; Schierup, Mikkel Heide. / Determination of recombination in Mycoplasma hominis. I: Infect Genet Evol. 2002 ; Bind 1. s. 277-85.

Bibtex

@article{34a02300c72c11dbbee902004c4f4f50,
title = "Determination of recombination in Mycoplasma hominis",
abstract = "Mycoplasma hominis has been previously described as a heterogeneous species, and in the present study intraspecies diversity of 20 M. hominis isolates from different individuals was analyzed using parts of the unlinked gyrase B (gyrB), elongation factor Tu (tuf), SRalpha homolog (ftsY), hitB-hitL, excinuclease ABC subunit A (uvrA) and glyceraldehyde-3-phosphate dehydrogenase (gap) genes. The level of variability of these M. hominis genes was low compared with the housekeeping genes from Helicobacter pylori and Neisseria meningitidis, but only few M. hominis isolates had identical sequences in all genes indicating the presence of recombination. In order to test for intergenic recombination, phylogenetic trees were reconstructed for each of the genes but no well-supported bifurcating phylogenetic trees could be obtained. The genes were tested for intragenic recombination using the correlation between linkage disequilibrium and distance between the segregating sites, by the homoplasy ratio (H ratio), and by compatibility matrices. The gap gene showed well-supported evidence for high levels of recombination, whereas recombination was less frequent and not significant within the other genes. The analysis revealed intergenic and intragenic recombination in M. hominis and this may explain the high intraspecies variability. The results obtained in the present study may be of importance for future population studies of Mycoplasma species.",
author = "Jacobsen, {Iben S{\o}gaard} and Thomas Boesen and Tina Mygind and Renata Melkova and Svend Birkelund and Gunna Christiansen and Schierup, {Mikkel Heide}",
year = "2002",
language = "English",
volume = "1",
pages = "277--85",
journal = "Infect Genet Evol",

}

RIS

TY - JOUR

T1 - Determination of recombination in Mycoplasma hominis

AU - Jacobsen, Iben Søgaard

AU - Boesen, Thomas

AU - Mygind, Tina

AU - Melkova, Renata

AU - Birkelund, Svend

AU - Christiansen, Gunna

AU - Schierup, Mikkel Heide

PY - 2002

Y1 - 2002

N2 - Mycoplasma hominis has been previously described as a heterogeneous species, and in the present study intraspecies diversity of 20 M. hominis isolates from different individuals was analyzed using parts of the unlinked gyrase B (gyrB), elongation factor Tu (tuf), SRalpha homolog (ftsY), hitB-hitL, excinuclease ABC subunit A (uvrA) and glyceraldehyde-3-phosphate dehydrogenase (gap) genes. The level of variability of these M. hominis genes was low compared with the housekeeping genes from Helicobacter pylori and Neisseria meningitidis, but only few M. hominis isolates had identical sequences in all genes indicating the presence of recombination. In order to test for intergenic recombination, phylogenetic trees were reconstructed for each of the genes but no well-supported bifurcating phylogenetic trees could be obtained. The genes were tested for intragenic recombination using the correlation between linkage disequilibrium and distance between the segregating sites, by the homoplasy ratio (H ratio), and by compatibility matrices. The gap gene showed well-supported evidence for high levels of recombination, whereas recombination was less frequent and not significant within the other genes. The analysis revealed intergenic and intragenic recombination in M. hominis and this may explain the high intraspecies variability. The results obtained in the present study may be of importance for future population studies of Mycoplasma species.

AB - Mycoplasma hominis has been previously described as a heterogeneous species, and in the present study intraspecies diversity of 20 M. hominis isolates from different individuals was analyzed using parts of the unlinked gyrase B (gyrB), elongation factor Tu (tuf), SRalpha homolog (ftsY), hitB-hitL, excinuclease ABC subunit A (uvrA) and glyceraldehyde-3-phosphate dehydrogenase (gap) genes. The level of variability of these M. hominis genes was low compared with the housekeeping genes from Helicobacter pylori and Neisseria meningitidis, but only few M. hominis isolates had identical sequences in all genes indicating the presence of recombination. In order to test for intergenic recombination, phylogenetic trees were reconstructed for each of the genes but no well-supported bifurcating phylogenetic trees could be obtained. The genes were tested for intragenic recombination using the correlation between linkage disequilibrium and distance between the segregating sites, by the homoplasy ratio (H ratio), and by compatibility matrices. The gap gene showed well-supported evidence for high levels of recombination, whereas recombination was less frequent and not significant within the other genes. The analysis revealed intergenic and intragenic recombination in M. hominis and this may explain the high intraspecies variability. The results obtained in the present study may be of importance for future population studies of Mycoplasma species.

M3 - Journal article

VL - 1

SP - 277

EP - 285

JO - Infect Genet Evol

JF - Infect Genet Evol

ER -