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Determination of primary microRNA processing in clinical samples by targeted pri-miR-sequencing

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Determination of primary microRNA processing in clinical samples by targeted pri-miR-sequencing. / Conrad, Thomas; Ntini, Evgenia; Lang, Benjamin; Cozzuto, Luca; Andersen, Jesper B.; Marquardt, Jens U.; Ponomarenko, Julia; Tartaglia, Gian Gaetano; Ørom, Ulf A. Vang.

I: RNA, Bind 26, Nr. 11, 11.2020, s. 1726-1730.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Conrad, T, Ntini, E, Lang, B, Cozzuto, L, Andersen, JB, Marquardt, JU, Ponomarenko, J, Tartaglia, GG & Ørom, UAV 2020, 'Determination of primary microRNA processing in clinical samples by targeted pri-miR-sequencing', RNA, bind 26, nr. 11, s. 1726-1730. https://doi.org/10.1261/rna.076240.120

APA

Conrad, T., Ntini, E., Lang, B., Cozzuto, L., Andersen, J. B., Marquardt, J. U., Ponomarenko, J., Tartaglia, G. G., & Ørom, U. A. V. (2020). Determination of primary microRNA processing in clinical samples by targeted pri-miR-sequencing. RNA, 26(11), 1726-1730. https://doi.org/10.1261/rna.076240.120

CBE

Conrad T, Ntini E, Lang B, Cozzuto L, Andersen JB, Marquardt JU, Ponomarenko J, Tartaglia GG, Ørom UAV. 2020. Determination of primary microRNA processing in clinical samples by targeted pri-miR-sequencing. RNA. 26(11):1726-1730. https://doi.org/10.1261/rna.076240.120

MLA

Vancouver

Conrad T, Ntini E, Lang B, Cozzuto L, Andersen JB, Marquardt JU o.a. Determination of primary microRNA processing in clinical samples by targeted pri-miR-sequencing. RNA. 2020 nov;26(11):1726-1730. https://doi.org/10.1261/rna.076240.120

Author

Conrad, Thomas ; Ntini, Evgenia ; Lang, Benjamin ; Cozzuto, Luca ; Andersen, Jesper B. ; Marquardt, Jens U. ; Ponomarenko, Julia ; Tartaglia, Gian Gaetano ; Ørom, Ulf A. Vang. / Determination of primary microRNA processing in clinical samples by targeted pri-miR-sequencing. I: RNA. 2020 ; Bind 26, Nr. 11. s. 1726-1730.

Bibtex

@article{58fb58158ef7471c812a05eac313746d,
title = "Determination of primary microRNA processing in clinical samples by targeted pri-miR-sequencing",
abstract = "MicroRNA expression is important for gene regulation and deregulated microRNA expression is often observed in diseases such as cancer. The processing of primary microRNA transcripts is an important regulatory step in microRNA biogenesis. Due to low expression level and association with chromatin, primary microRNAs are challenging to study in clinical samples where input material is limited. Here, we present a high-sensitivity targeted method to determine processing efficiency of several hundred primary microRNAs from total RNA that requires relatively few RNA sequencing reads. We validate the method using RNA from HeLa cells and show the applicability to clinical samples by analyzing RNA from normal liver and hepatocellular carcinoma. We identify 24 primary microRNAs with significant changes in processing efficiency from normal liver to hepatocellular carcinoma, among those the highly expressed miRNA-122 and miRNA-21, demonstrating that differential processing of primary microRNAs is occurring and could be involved in disease.With our method presented here we provide means to study pri-miRNA processing in disease from clinical samples. ",
keywords = "Clinical samples, HCC, Liver, miRNA biogenesis, Primary miRNAs, RNA sequencing",
author = "Thomas Conrad and Evgenia Ntini and Benjamin Lang and Luca Cozzuto and Andersen, {Jesper B.} and Marquardt, {Jens U.} and Julia Ponomarenko and Tartaglia, {Gian Gaetano} and {\O}rom, {Ulf A. Vang}",
year = "2020",
month = nov,
doi = "10.1261/rna.076240.120",
language = "English",
volume = "26",
pages = "1726--1730",
journal = "RNA",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
number = "11",

}

RIS

TY - JOUR

T1 - Determination of primary microRNA processing in clinical samples by targeted pri-miR-sequencing

AU - Conrad, Thomas

AU - Ntini, Evgenia

AU - Lang, Benjamin

AU - Cozzuto, Luca

AU - Andersen, Jesper B.

AU - Marquardt, Jens U.

AU - Ponomarenko, Julia

AU - Tartaglia, Gian Gaetano

AU - Ørom, Ulf A. Vang

PY - 2020/11

Y1 - 2020/11

N2 - MicroRNA expression is important for gene regulation and deregulated microRNA expression is often observed in diseases such as cancer. The processing of primary microRNA transcripts is an important regulatory step in microRNA biogenesis. Due to low expression level and association with chromatin, primary microRNAs are challenging to study in clinical samples where input material is limited. Here, we present a high-sensitivity targeted method to determine processing efficiency of several hundred primary microRNAs from total RNA that requires relatively few RNA sequencing reads. We validate the method using RNA from HeLa cells and show the applicability to clinical samples by analyzing RNA from normal liver and hepatocellular carcinoma. We identify 24 primary microRNAs with significant changes in processing efficiency from normal liver to hepatocellular carcinoma, among those the highly expressed miRNA-122 and miRNA-21, demonstrating that differential processing of primary microRNAs is occurring and could be involved in disease.With our method presented here we provide means to study pri-miRNA processing in disease from clinical samples.

AB - MicroRNA expression is important for gene regulation and deregulated microRNA expression is often observed in diseases such as cancer. The processing of primary microRNA transcripts is an important regulatory step in microRNA biogenesis. Due to low expression level and association with chromatin, primary microRNAs are challenging to study in clinical samples where input material is limited. Here, we present a high-sensitivity targeted method to determine processing efficiency of several hundred primary microRNAs from total RNA that requires relatively few RNA sequencing reads. We validate the method using RNA from HeLa cells and show the applicability to clinical samples by analyzing RNA from normal liver and hepatocellular carcinoma. We identify 24 primary microRNAs with significant changes in processing efficiency from normal liver to hepatocellular carcinoma, among those the highly expressed miRNA-122 and miRNA-21, demonstrating that differential processing of primary microRNAs is occurring and could be involved in disease.With our method presented here we provide means to study pri-miRNA processing in disease from clinical samples.

KW - Clinical samples

KW - HCC

KW - Liver

KW - miRNA biogenesis

KW - Primary miRNAs

KW - RNA sequencing

UR - http://www.scopus.com/inward/record.url?scp=85093522395&partnerID=8YFLogxK

U2 - 10.1261/rna.076240.120

DO - 10.1261/rna.076240.120

M3 - Journal article

C2 - 32669295

AN - SCOPUS:85093522395

VL - 26

SP - 1726

EP - 1730

JO - RNA

JF - RNA

SN - 1355-8382

IS - 11

ER -