Detection of proteolytic signatures for Parkinson's disease

Peter Lüttge Jordal; Thomas F. Dyrlund; Kristian Winge; Martin R. Larsen; Erik H. Danielsen; James A. Wells; Daniel E. Otzen; Jan J. Enghild

doi:10.2217/fnl.16.3

Detection of proteolytic signatures for Parkinson's disease

Peter Lüttge Jordal, Thomas F. Dyrlund, Kristian Winge, Martin R. Larsen, Erik H. Danielsen, James A. Wells, Daniel E. Otzen, Jan J. Enghild^*

^*Corresponding author af dette arbejde

Institut for Molekylærbiologi og Genetik - Plantemolekylærbiologi

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Abstract

Aim: To investigate if idiopathic Parkinson's disease (IPD) is associated with distinct proteolytic signatures relative to non-neurodegenerative controls (NND) and patients with multiple system atrophy (MSA). Materials & methods: A subtiligase-based N-terminomics screening method was exploited for semiquantitative comparison of protein N-termini in cerebrospinal fluid for pooled samples of IPD (n = 6) and NND (n = 8) individuals. Subsequently, targeted selected reaction monitoring mass spectrometry measured the relative concentration of the proteolytic signature peptides in individual IPD (n = 22), NND (n = 11) and MSA (n = 18) samples. Results: The discovery screen detected 300 N-termini for 156 proteins. Selected reaction monitoring analysis revealed that two of these peptides differentiate IPD from NND while three peptides differentiate IPD from MSA. Conclusion: IPD is associated with distinct proteolytic signatures.

Originalsprog	Engelsk
Tidsskrift	Future Neurology
Vol/bind	11
Nummer	1
Sider (fra-til)	15-32
Antal sider	18
ISSN	1479-6708
DOI	https://doi.org/10.2217/fnl.16.3
Status	Udgivet - 1 feb. 2016

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Citationsformater

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title = "Detection of proteolytic signatures for Parkinson's disease",

abstract = "Aim: To investigate if idiopathic Parkinson's disease (IPD) is associated with distinct proteolytic signatures relative to non-neurodegenerative controls (NND) and patients with multiple system atrophy (MSA). Materials & methods: A subtiligase-based N-terminomics screening method was exploited for semiquantitative comparison of protein N-termini in cerebrospinal fluid for pooled samples of IPD (n = 6) and NND (n = 8) individuals. Subsequently, targeted selected reaction monitoring mass spectrometry measured the relative concentration of the proteolytic signature peptides in individual IPD (n = 22), NND (n = 11) and MSA (n = 18) samples. Results: The discovery screen detected 300 N-termini for 156 proteins. Selected reaction monitoring analysis revealed that two of these peptides differentiate IPD from NND while three peptides differentiate IPD from MSA. Conclusion: IPD is associated with distinct proteolytic signatures.",

keywords = "cerebrospinal fluid, N-terminomics, Parkinson's disease",

author = "Jordal, {Peter L{\"u}ttge} and Dyrlund, {Thomas F.} and Kristian Winge and Larsen, {Martin R.} and Danielsen, {Erik H.} and Wells, {James A.} and Otzen, {Daniel E.} and Enghild, {Jan J.}",

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day = "1",

doi = "10.2217/fnl.16.3",

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T1 - Detection of proteolytic signatures for Parkinson's disease

AU - Jordal, Peter Lüttge

AU - Dyrlund, Thomas F.

AU - Winge, Kristian

AU - Larsen, Martin R.

AU - Danielsen, Erik H.

AU - Wells, James A.

AU - Otzen, Daniel E.

AU - Enghild, Jan J.

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Aim: To investigate if idiopathic Parkinson's disease (IPD) is associated with distinct proteolytic signatures relative to non-neurodegenerative controls (NND) and patients with multiple system atrophy (MSA). Materials & methods: A subtiligase-based N-terminomics screening method was exploited for semiquantitative comparison of protein N-termini in cerebrospinal fluid for pooled samples of IPD (n = 6) and NND (n = 8) individuals. Subsequently, targeted selected reaction monitoring mass spectrometry measured the relative concentration of the proteolytic signature peptides in individual IPD (n = 22), NND (n = 11) and MSA (n = 18) samples. Results: The discovery screen detected 300 N-termini for 156 proteins. Selected reaction monitoring analysis revealed that two of these peptides differentiate IPD from NND while three peptides differentiate IPD from MSA. Conclusion: IPD is associated with distinct proteolytic signatures.

AB - Aim: To investigate if idiopathic Parkinson's disease (IPD) is associated with distinct proteolytic signatures relative to non-neurodegenerative controls (NND) and patients with multiple system atrophy (MSA). Materials & methods: A subtiligase-based N-terminomics screening method was exploited for semiquantitative comparison of protein N-termini in cerebrospinal fluid for pooled samples of IPD (n = 6) and NND (n = 8) individuals. Subsequently, targeted selected reaction monitoring mass spectrometry measured the relative concentration of the proteolytic signature peptides in individual IPD (n = 22), NND (n = 11) and MSA (n = 18) samples. Results: The discovery screen detected 300 N-termini for 156 proteins. Selected reaction monitoring analysis revealed that two of these peptides differentiate IPD from NND while three peptides differentiate IPD from MSA. Conclusion: IPD is associated with distinct proteolytic signatures.

KW - cerebrospinal fluid

KW - N-terminomics

KW - Parkinson's disease

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U2 - 10.2217/fnl.16.3

DO - 10.2217/fnl.16.3

M3 - Journal article

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SN - 1479-6708

VL - 11

SP - 15

EP - 32

JO - Future Neurology

JF - Future Neurology

IS - 1

ER -

Detection of proteolytic signatures for Parkinson's disease

Abstract

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