Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11

Jun-Young Kim; Srikanta Sahu; Yin-Hoe Yau; Xu Wang; Susana Geifman Shochat; Per Halkjær Nielsen; Morten Simonsen Dueholm; Daniel E Otzen; Jungyeol Lee; May Margarette Salido Delos Santos; Joey Kuok Hoong Yam; Nam-Young Kang; Sung-Jin Park; Hawyoung Kwon; Thomas William Seviour; Liang Yang; Michael Givskov; Young-Tae Chang

doi:10.1021/jacs.5b11357

Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11

Jun-Young Kim, Srikanta Sahu, Yin-Hoe Yau, Xu Wang, Susana Geifman Shochat, Per Halkjær Nielsen, Morten Simonsen Dueholm, Daniel E Otzen, Jungyeol Lee, May Margarette Salido Delos Santos, Joey Kuok Hoong Yam, Nam-Young Kang, Sung-Jin Park, Hawyoung Kwon, Thomas William Seviour, Liang Yang, Michael Givskov, Young-Tae Chang

Interdisciplinary Nanoscience Center - INANO-MBG, iNANO-huset

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

77 Citationer (Scopus)

Abstract

Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods which fail to detect non-culturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (Compound of Designation Yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a Diversity Oriented Fluorescent Library Approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.

Originalsprog	Engelsk
Tidsskrift	Journal of the American Chemical Society
Vol/bind	138
Nummer	1
Sider (fra-til)	402-407
Antal sider	6
ISSN	0002-7863
DOI	https://doi.org/10.1021/jacs.5b11357
Status	Udgivet - 13 jan. 2016

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Kim, J.-Y., Sahu, S., Yau, Y.-H., Wang, X., Shochat, S. G., Nielsen, P. H., Dueholm, M. S., Otzen, D. E., Lee, J., Delos Santos, M. M. S., Yam, J. K. H., Kang, N.-Y., Park, S.-J., Kwon, H., Seviour, T. W., Yang, L., Givskov, M., & Chang, Y.-T. (2016). Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11. Journal of the American Chemical Society, 138(1), 402-407. https://doi.org/10.1021/jacs.5b11357

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title = "Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11",

abstract = "Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods which fail to detect non-culturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (Compound of Designation Yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a Diversity Oriented Fluorescent Library Approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.",

author = "Jun-Young Kim and Srikanta Sahu and Yin-Hoe Yau and Xu Wang and Shochat, {Susana Geifman} and Nielsen, {Per Halkj{\ae}r} and Dueholm, {Morten Simonsen} and Otzen, {Daniel E} and Jungyeol Lee and {Delos Santos}, {May Margarette Salido} and Yam, {Joey Kuok Hoong} and Nam-Young Kang and Sung-Jin Park and Hawyoung Kwon and Seviour, {Thomas William} and Liang Yang and Michael Givskov and Young-Tae Chang",

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doi = "10.1021/jacs.5b11357",

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Kim, J-Y, Sahu, S, Yau, Y-H, Wang, X, Shochat, SG, Nielsen, PH, Dueholm, MS, Otzen, DE, Lee, J, Delos Santos, MMS, Yam, JKH, Kang, N-Y, Park, S-J, Kwon, H, Seviour, TW, Yang, L, Givskov, M & Chang, Y-T 2016, 'Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11', Journal of the American Chemical Society, bind 138, nr. 1, s. 402-407. https://doi.org/10.1021/jacs.5b11357

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T1 - Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11

AU - Kim, Jun-Young

AU - Sahu, Srikanta

AU - Yau, Yin-Hoe

AU - Wang, Xu

AU - Shochat, Susana Geifman

AU - Nielsen, Per Halkjær

AU - Dueholm, Morten Simonsen

AU - Otzen, Daniel E

AU - Lee, Jungyeol

AU - Delos Santos, May Margarette Salido

AU - Yam, Joey Kuok Hoong

AU - Kang, Nam-Young

AU - Park, Sung-Jin

AU - Kwon, Hawyoung

AU - Seviour, Thomas William

AU - Yang, Liang

AU - Givskov, Michael

AU - Chang, Young-Tae

PY - 2016/1/13

Y1 - 2016/1/13

N2 - Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods which fail to detect non-culturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (Compound of Designation Yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a Diversity Oriented Fluorescent Library Approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.

AB - Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods which fail to detect non-culturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (Compound of Designation Yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a Diversity Oriented Fluorescent Library Approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.

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U2 - 10.1021/jacs.5b11357

DO - 10.1021/jacs.5b11357

M3 - Journal article

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SN - 0002-7863

VL - 138

SP - 402

EP - 407

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

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Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11

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