@article{fb5b1c6714654cfc9f39638aa86bc27b,
title = "Detection of magnetic field effects by confocal microscopy",
abstract = "Certain pairs of paramagnetic species generated under conservation of total spin angular momentum are known to undergo magnetosensitive processes. Two prominent examples of systems exhibiting these so-called magnetic field effects (MFEs) are photogenerated radical pairs created from either singlet or triplet molecular precursors, and pairs of triplet states generated by singlet fission. Here, we showcase confocal microscopy as a powerful technique for the investigation of such phenomena. We first characterise the instrument by studying the field-sensitive chemistry of two systems in solution: radical pairs formed in a cryptochrome protein and the flavin mononucleotide/hen egg-white lysozyme model system. We then extend these studies to single crystals. Firstly, we report temporally and spatially resolved MFEs in flavin-doped lysozyme single crystals. Anisotropic magnetic field effects are then reported in tetracene single crystals. Finally, we discuss the future applications of confocal microscopy for the study of magnetosensitive processes with a particular focus on the cryptochrome-based chemical compass believed to lie at the heart of animal magnetoreception.",
author = "Victoire D{\'e}jean and Marcin Konowalczyk and Jamie Gravell and Golesworthy, {Matthew J.} and Catlin Gunn and Nils Pompe and {Foster Vander Elst}, Olivia and Tan, {Ke Jie} and Mark Oxborrow and Aarts, {Dirk G.A.L.} and Mackenzie, {Stuart R.} and Timmel, {Christiane R.}",
note = "Funding Information: We thank the electronic and mechanical workshops (Department of Chemistry, University of Oxford), and especially Neville Baker, Philip Hurst and Timothy Powell, for the design and construction of the magnetic eld apparatus. We are grateful for Dr Charlotte Dodson's assistance with protein expression and purication. We thank Dr Kirsten Christensen and Prof. Susan Lea for the X-ray crystallographic measurements of HEWL crystals. We are grateful to Mr Gabriel Moise for insightful discussions on spin dynamics. Victoire D{\'e}jean is indebted to the Clarendon Fund for her doctoral scholarship. Marcin Konowalczyk is thankful to the Air Force Office of Scientic Research (AFOSR, USAF award number FA9550-14-1-0095) for funding of their doctoral studies. Jamie Gravell is funded by the European Research Council (ERC) as part of the QuantumBirds project under the European Union's Horizon 2020 research and innovation programme (Grant agreement No. 810002). Matthew Golesworthy gratefully acknowledges the support of the Biotechnology and Biological Sciences Research Council (BBSRC – grant number BB/M011224/ 1) and the Clarendon Fund. Dr Ke-Jie Tan gratefully acknowledges support through an A*STAR post-doctoral fellowship provided by Singapore's Agency for Science Technology & Research. Dr M. Oxborrow's work was supported by EPSRC contract EP/K037390/1 (“Manufacturing Routes for Organic Room-Temperature MASER”). Molecular graphics were created with UCSF Chimera, developed by the Resource for Biocomput-ing, Visualization, and Informatics at the University of California, San Francisco, with support from NIH P41-GM103311. Publisher Copyright: {\textcopyright} 2020 The Royal Society of Chemistry.",
year = "2020",
month = aug,
day = "14",
doi = "10.1039/d0sc01986k",
language = "English",
volume = "11",
pages = "7772--7781",
journal = "Chemical Science",
issn = "2041-6520",
publisher = "Royal Society of Chemistry",
number = "30",
}