Detection methods targeting the positive- and negative-sense RNA transcripts from plus-stranded RNA viruses

TY - JOUR

T1 - Detection methods targeting the positive- and negative-sense RNA transcripts from plus-stranded RNA viruses

AU - Warncke, Signe Ravn

AU - Knudsen, Charlotte Rohde

N1 - Publisher Copyright: © 2021 The Authors. APMIS published by John Wiley & Sons Ltd on behalf of Scandinavian Societies for Medical Microbiology and Pathology.

PY - 2022/5

Y1 - 2022/5

N2 - The largest group of viruses in the Baltimore classification system comprises viruses with a positive-sense, single-stranded RNA genome. Once the viral genome is released into the cytoplasm of a specific host cell following virus entry, it functions directly as an mRNA, and the virus-encoded proteins that are essential for genome replication are produced by the translation apparatus of the host cell. The positive-sense genome is replicated in two stages, initially the positive strand is copied to make a negative-sense RNA, which then functions as the template for transcription of many new positive-sense genomes. Virus infections can be detected at different stages throughout the infection cycle for diagnostic and scientific purposes. Here, the advantages and disadvantages of some of the relevant methods for genome detection will be briefly reviewed with special emphasis on techniques allowing strand-specific RNA detection. Furthermore, tools of the future are considered.

AB - The largest group of viruses in the Baltimore classification system comprises viruses with a positive-sense, single-stranded RNA genome. Once the viral genome is released into the cytoplasm of a specific host cell following virus entry, it functions directly as an mRNA, and the virus-encoded proteins that are essential for genome replication are produced by the translation apparatus of the host cell. The positive-sense genome is replicated in two stages, initially the positive strand is copied to make a negative-sense RNA, which then functions as the template for transcription of many new positive-sense genomes. Virus infections can be detected at different stages throughout the infection cycle for diagnostic and scientific purposes. Here, the advantages and disadvantages of some of the relevant methods for genome detection will be briefly reviewed with special emphasis on techniques allowing strand-specific RNA detection. Furthermore, tools of the future are considered.

KW - genome

KW - methods

KW - Plus-stranded RNA viruses

KW - replication

KW - RT-PCR

KW - strand-specific detection

UR - https://www.scopus.com/pages/publications/85123577407

U2 - 10.1111/apm.13202

DO - 10.1111/apm.13202

M3 - Journal article

C2 - 34939239

AN - SCOPUS:85123577407

SN - 0903-4641

VL - 130

SP - 284

EP - 292

JO - APMIS

JF - APMIS

IS - 5

ER -