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Derepression of the plant Chromovirus LORE1 induces germline transposition in regenerated plants

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Derepression of the plant Chromovirus LORE1 induces germline transposition in regenerated plants. / Fukai, Eigo; Umehara, Yosuke; Sato, Shusei; Endo, Makoto; Kouchi, Hiroshi; Hayashi, Makoto; Stougaard, Jens; Hirochika, Hirohiko.

I: P L o S Genetics, Bind 6, Nr. 3, 01.01.2010, s. e1000868.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Fukai, E, Umehara, Y, Sato, S, Endo, M, Kouchi, H, Hayashi, M, Stougaard, J & Hirochika, H 2010, 'Derepression of the plant Chromovirus LORE1 induces germline transposition in regenerated plants', P L o S Genetics, bind 6, nr. 3, s. e1000868. https://doi.org/10.1371/journal.pgen.1000868

APA

Fukai, E., Umehara, Y., Sato, S., Endo, M., Kouchi, H., Hayashi, M., Stougaard, J., & Hirochika, H. (2010). Derepression of the plant Chromovirus LORE1 induces germline transposition in regenerated plants. P L o S Genetics, 6(3), e1000868. https://doi.org/10.1371/journal.pgen.1000868

CBE

Fukai E, Umehara Y, Sato S, Endo M, Kouchi H, Hayashi M, Stougaard J, Hirochika H. 2010. Derepression of the plant Chromovirus LORE1 induces germline transposition in regenerated plants. P L o S Genetics. 6(3):e1000868. https://doi.org/10.1371/journal.pgen.1000868

MLA

Vancouver

Fukai E, Umehara Y, Sato S, Endo M, Kouchi H, Hayashi M o.a. Derepression of the plant Chromovirus LORE1 induces germline transposition in regenerated plants. P L o S Genetics. 2010 jan 1;6(3):e1000868. https://doi.org/10.1371/journal.pgen.1000868

Author

Fukai, Eigo ; Umehara, Yosuke ; Sato, Shusei ; Endo, Makoto ; Kouchi, Hiroshi ; Hayashi, Makoto ; Stougaard, Jens ; Hirochika, Hirohiko. / Derepression of the plant Chromovirus LORE1 induces germline transposition in regenerated plants. I: P L o S Genetics. 2010 ; Bind 6, Nr. 3. s. e1000868.

Bibtex

@article{f20d69ce69ef4ab796e4a9189d4ef194,
title = "Derepression of the plant Chromovirus LORE1 induces germline transposition in regenerated plants",
abstract = "Transposable elements represent a large proportion of the eukaryotic genomes. Long Terminal Repeat (LTR) retrotransposons are very abundant and constitute the predominant family of transposable elements in plants. Recent studies have identified chromoviruses to be a widely distributed lineage of Gypsy elements. These elements contain chromodomains in their integrases, which suggests a preference for insertion into heterochromatin. In turn, this preference might have contributed to the patterning of heterochromatin observed in host genomes. Despite their potential importance for our understanding of plant genome dynamics and evolution, the regulatory mechanisms governing the behavior of chromoviruses and their activities remain largely uncharacterized. Here, we report a detailed analysis of the spatio-temporal activity of a plant chromovirus in the endogenous host. We examined LORE1a, a member of the endogenous chromovirus LORE1 family from the model legume Lotus japonicus. We found that this chromovirus is stochastically de-repressed in plant populations regenerated from de-differentiated cells and that LORE1a transposes in the male germline. Bisulfite sequencing of the 5' LTR and its surrounding region suggests that tissue culture induces a loss of epigenetic silencing of LORE1a. Since LTR promoter activity is pollen specific, as shown by the analysis of transgenic plants containing an LTR::GUS fusion, we conclude that male germline-specific LORE1a transposition in pollen grains is controlled transcriptionally by its own cis-elements. New insertion sites of LORE1a copies were frequently found in genic regions and show no strong insertional preferences. These distinctive novel features of LORE1 indicate that this chromovirus has considerable potential for generating genetic and epigenetic diversity in the host plant population. Our results also define conditions for the use of LORE1a as a genetic tool.",
keywords = "Alu Elements, Chromosome Mapping, Cytosine, DNA Methylation, DNA Transposable Elements, Genetic Variation, Germ Cells, Plant, Lotus, Mutagenesis, Insertional, Organ Specificity, Plant Viruses, Promoter Regions, Genetic, Regeneration, Terminal Repeat Sequences, Transcription, Genetic",
author = "Eigo Fukai and Yosuke Umehara and Shusei Sato and Makoto Endo and Hiroshi Kouchi and Makoto Hayashi and Jens Stougaard and Hirohiko Hirochika",
year = "2010",
month = jan,
day = "1",
doi = "10.1371/journal.pgen.1000868",
language = "English",
volume = "6",
pages = "e1000868",
journal = "P L o S Genetics",
issn = "1553-7390",
publisher = "public library of science",
number = "3",

}

RIS

TY - JOUR

T1 - Derepression of the plant Chromovirus LORE1 induces germline transposition in regenerated plants

AU - Fukai, Eigo

AU - Umehara, Yosuke

AU - Sato, Shusei

AU - Endo, Makoto

AU - Kouchi, Hiroshi

AU - Hayashi, Makoto

AU - Stougaard, Jens

AU - Hirochika, Hirohiko

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Transposable elements represent a large proportion of the eukaryotic genomes. Long Terminal Repeat (LTR) retrotransposons are very abundant and constitute the predominant family of transposable elements in plants. Recent studies have identified chromoviruses to be a widely distributed lineage of Gypsy elements. These elements contain chromodomains in their integrases, which suggests a preference for insertion into heterochromatin. In turn, this preference might have contributed to the patterning of heterochromatin observed in host genomes. Despite their potential importance for our understanding of plant genome dynamics and evolution, the regulatory mechanisms governing the behavior of chromoviruses and their activities remain largely uncharacterized. Here, we report a detailed analysis of the spatio-temporal activity of a plant chromovirus in the endogenous host. We examined LORE1a, a member of the endogenous chromovirus LORE1 family from the model legume Lotus japonicus. We found that this chromovirus is stochastically de-repressed in plant populations regenerated from de-differentiated cells and that LORE1a transposes in the male germline. Bisulfite sequencing of the 5' LTR and its surrounding region suggests that tissue culture induces a loss of epigenetic silencing of LORE1a. Since LTR promoter activity is pollen specific, as shown by the analysis of transgenic plants containing an LTR::GUS fusion, we conclude that male germline-specific LORE1a transposition in pollen grains is controlled transcriptionally by its own cis-elements. New insertion sites of LORE1a copies were frequently found in genic regions and show no strong insertional preferences. These distinctive novel features of LORE1 indicate that this chromovirus has considerable potential for generating genetic and epigenetic diversity in the host plant population. Our results also define conditions for the use of LORE1a as a genetic tool.

AB - Transposable elements represent a large proportion of the eukaryotic genomes. Long Terminal Repeat (LTR) retrotransposons are very abundant and constitute the predominant family of transposable elements in plants. Recent studies have identified chromoviruses to be a widely distributed lineage of Gypsy elements. These elements contain chromodomains in their integrases, which suggests a preference for insertion into heterochromatin. In turn, this preference might have contributed to the patterning of heterochromatin observed in host genomes. Despite their potential importance for our understanding of plant genome dynamics and evolution, the regulatory mechanisms governing the behavior of chromoviruses and their activities remain largely uncharacterized. Here, we report a detailed analysis of the spatio-temporal activity of a plant chromovirus in the endogenous host. We examined LORE1a, a member of the endogenous chromovirus LORE1 family from the model legume Lotus japonicus. We found that this chromovirus is stochastically de-repressed in plant populations regenerated from de-differentiated cells and that LORE1a transposes in the male germline. Bisulfite sequencing of the 5' LTR and its surrounding region suggests that tissue culture induces a loss of epigenetic silencing of LORE1a. Since LTR promoter activity is pollen specific, as shown by the analysis of transgenic plants containing an LTR::GUS fusion, we conclude that male germline-specific LORE1a transposition in pollen grains is controlled transcriptionally by its own cis-elements. New insertion sites of LORE1a copies were frequently found in genic regions and show no strong insertional preferences. These distinctive novel features of LORE1 indicate that this chromovirus has considerable potential for generating genetic and epigenetic diversity in the host plant population. Our results also define conditions for the use of LORE1a as a genetic tool.

KW - Alu Elements

KW - Chromosome Mapping

KW - Cytosine

KW - DNA Methylation

KW - DNA Transposable Elements

KW - Genetic Variation

KW - Germ Cells, Plant

KW - Lotus

KW - Mutagenesis, Insertional

KW - Organ Specificity

KW - Plant Viruses

KW - Promoter Regions, Genetic

KW - Regeneration

KW - Terminal Repeat Sequences

KW - Transcription, Genetic

U2 - 10.1371/journal.pgen.1000868

DO - 10.1371/journal.pgen.1000868

M3 - Journal article

C2 - 20221264

VL - 6

SP - e1000868

JO - P L o S Genetics

JF - P L o S Genetics

SN - 1553-7390

IS - 3

ER -