TY - JOUR
T1 - Dataset on gene expression profiling of multiple murine hair follicle populations
AU - Gunnarsson, Anders Patrik
AU - Christensen, Rikke
AU - Li, Jian
AU - Jensen, Uffe Birk
PY - 2016/12
Y1 - 2016/12
N2 - The murine hair follicle contains several different keratinocyte progenitor populations within its compartments. By using antibodies against CD34, Itgα6, Sca-1 and Plet-1, we have isolated eight populations and compared their Krt10 and Krt14 expressions using fluorescence microscopy. This improved panel was used in our associated article doi:10.1016/j.scr.2016.06.002 (A.P. Gunnarsson, R. Christensen, J. Li, U.B. Jensen, 2016) [1] and the present dataset describes the basic controls for the FACS. We also used imaging flow cytometry to visualize the identified populations as control. A more detailed analysis of the global gene expression profiling is presented, focusing on the pilosebaceous unit. Murine whole-mounts were stained for heat shock protein Hspa2, which is exclusively expressed by keratinocytes with low or no expression of the four selection markers (IRK). Whole-mount labeling was also conducted to visualize Krt79 and Plet-1 coexpression within the hair follicle and quantification on the distribution of Krt79 positive keratinocytes is presented.
AB - The murine hair follicle contains several different keratinocyte progenitor populations within its compartments. By using antibodies against CD34, Itgα6, Sca-1 and Plet-1, we have isolated eight populations and compared their Krt10 and Krt14 expressions using fluorescence microscopy. This improved panel was used in our associated article doi:10.1016/j.scr.2016.06.002 (A.P. Gunnarsson, R. Christensen, J. Li, U.B. Jensen, 2016) [1] and the present dataset describes the basic controls for the FACS. We also used imaging flow cytometry to visualize the identified populations as control. A more detailed analysis of the global gene expression profiling is presented, focusing on the pilosebaceous unit. Murine whole-mounts were stained for heat shock protein Hspa2, which is exclusively expressed by keratinocytes with low or no expression of the four selection markers (IRK). Whole-mount labeling was also conducted to visualize Krt79 and Plet-1 coexpression within the hair follicle and quantification on the distribution of Krt79 positive keratinocytes is presented.
KW - Journal Article
UR - http://www.scopus.com/inward/record.url?scp=84979634137&partnerID=8YFLogxK
U2 - 10.1016/j.dib.2016.08.063
DO - 10.1016/j.dib.2016.08.063
M3 - Journal article
C2 - 27672671
SN - 2352-3409
VL - 9
SP - 328
EP - 334
JO - Data in Brief
JF - Data in Brief
ER -