Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549. / Foldbjerg, Rasmus; Dang, Duy Anh; Autrup, Herman.

I: Archives of Toxicology, Bind 85, Nr. 7, 07.2011, s. 743-750.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

APA

CBE

MLA

Vancouver

Author

Bibtex

@article{fe3707c09f9f11df8c1a000ea68e967b,
title = "Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549",
abstract = "Nanomaterials, especially silver nanoparticles (Ag NPs), are used in a rapidly increasing number of commercial products. Accordingly, the hazards associated with human exposure to nanomaterials should be investigated to facilitate the risk assessment process. A potential route of exposure to NPs is through the respiratory system. In the present study, we investigated the effects of well-characterized PVP-coated Ag NPs and silver ions (Ag+) in the human, alveolar cell line, A549. Dose-dependent cellular toxicity caused by Ag NPs and Ag+ was demonstrated by the MTT and annexin V/propidium iodide assays, and evidence of Ag NP uptake could be measured indirectly by atomic absorption spectroscopy and flow cytometry. The cytotoxicity of both silver compounds was greatly decreased by pretreatment with the antioxidant, N-acetyl-cysteine, and a strong correlation between the levels of reactive oxygen species (ROS) and mitochondrial damage (r (s) = -0.8810; p = 0.0039) or early apoptosis (r (s) = 0.8857; p = 0.0188) was observed. DNA damage induced by ROS was detected as an increase in bulky DNA adducts by (32)P postlabeling after Ag NP exposure. The level of bulky DNA adducts was strongly correlated with the cellular ROS levels (r (s) = 0.8810, p = 0.0039) and could be inhibited by antioxidant pretreatment, suggesting Ag NPs as a mediator of ROS-induced genotoxicity.",
author = "Rasmus Foldbjerg and Dang, {Duy Anh} and Herman Autrup",
note = "Epub 2010 Apr 29",
year = "2011",
month = jul,
doi = "10.1007/s00204-010-0545-5",
language = "English",
volume = "85",
pages = "743--750",
journal = "Archives of Toxicology",
issn = "0340-5761",
publisher = "Springer",
number = "7",

}

RIS

TY - JOUR

T1 - Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549

AU - Foldbjerg, Rasmus

AU - Dang, Duy Anh

AU - Autrup, Herman

N1 - Epub 2010 Apr 29

PY - 2011/7

Y1 - 2011/7

N2 - Nanomaterials, especially silver nanoparticles (Ag NPs), are used in a rapidly increasing number of commercial products. Accordingly, the hazards associated with human exposure to nanomaterials should be investigated to facilitate the risk assessment process. A potential route of exposure to NPs is through the respiratory system. In the present study, we investigated the effects of well-characterized PVP-coated Ag NPs and silver ions (Ag+) in the human, alveolar cell line, A549. Dose-dependent cellular toxicity caused by Ag NPs and Ag+ was demonstrated by the MTT and annexin V/propidium iodide assays, and evidence of Ag NP uptake could be measured indirectly by atomic absorption spectroscopy and flow cytometry. The cytotoxicity of both silver compounds was greatly decreased by pretreatment with the antioxidant, N-acetyl-cysteine, and a strong correlation between the levels of reactive oxygen species (ROS) and mitochondrial damage (r (s) = -0.8810; p = 0.0039) or early apoptosis (r (s) = 0.8857; p = 0.0188) was observed. DNA damage induced by ROS was detected as an increase in bulky DNA adducts by (32)P postlabeling after Ag NP exposure. The level of bulky DNA adducts was strongly correlated with the cellular ROS levels (r (s) = 0.8810, p = 0.0039) and could be inhibited by antioxidant pretreatment, suggesting Ag NPs as a mediator of ROS-induced genotoxicity.

AB - Nanomaterials, especially silver nanoparticles (Ag NPs), are used in a rapidly increasing number of commercial products. Accordingly, the hazards associated with human exposure to nanomaterials should be investigated to facilitate the risk assessment process. A potential route of exposure to NPs is through the respiratory system. In the present study, we investigated the effects of well-characterized PVP-coated Ag NPs and silver ions (Ag+) in the human, alveolar cell line, A549. Dose-dependent cellular toxicity caused by Ag NPs and Ag+ was demonstrated by the MTT and annexin V/propidium iodide assays, and evidence of Ag NP uptake could be measured indirectly by atomic absorption spectroscopy and flow cytometry. The cytotoxicity of both silver compounds was greatly decreased by pretreatment with the antioxidant, N-acetyl-cysteine, and a strong correlation between the levels of reactive oxygen species (ROS) and mitochondrial damage (r (s) = -0.8810; p = 0.0039) or early apoptosis (r (s) = 0.8857; p = 0.0188) was observed. DNA damage induced by ROS was detected as an increase in bulky DNA adducts by (32)P postlabeling after Ag NP exposure. The level of bulky DNA adducts was strongly correlated with the cellular ROS levels (r (s) = 0.8810, p = 0.0039) and could be inhibited by antioxidant pretreatment, suggesting Ag NPs as a mediator of ROS-induced genotoxicity.

U2 - 10.1007/s00204-010-0545-5

DO - 10.1007/s00204-010-0545-5

M3 - Journal article

C2 - 20428844

VL - 85

SP - 743

EP - 750

JO - Archives of Toxicology

JF - Archives of Toxicology

SN - 0340-5761

IS - 7

ER -