TY - JOUR
T1 - Cytosolic Glutamine Synthetase is Important for Photosynthetic Efficiency and Water Use Efficiency in Potato as Revealed by High Throughput Sequencing QTL analysis
AU - Kaminski, Kacper Piotr
AU - Sørensen, Kirsten Kørup
AU - Andersen, Mathias Neumann
AU - Sønderkær, Mads
AU - Andersen, Mette Sondrup
AU - Kirk, Hanne Grethe
AU - Nielsen, Kåre Lehmann
PY - 2015
Y1 - 2015
N2 - Potato (Solanum tuberosum L.) closes its stomata at relatively low soil water deficits frequently encountered in normal field conditions resulting in unnecessary annual yield losses and extensive use of artificial irrigation. Therefore, unraveling the genetics underpinning variation in water use efficiency (WUE) of potato is important, but has been limited by technical difficulties in assessing the trait on individual plants and thus is poorly understood. In this study, a mapping population of potatoes has been robustly phenotyped, and considerable variation in WUE under well-watered conditions was observed. Two extreme WUE bulks of clones were identified and pools of genomic DNA from them as well as the parents were sequenced and mapped to reference potato genome. Following a novel data analysis approach, two highly resolved QTLs were found on chromosome 1 and 9. Interestingly, three genes encoding isoforms of cytosolic glutamine synthase were located in the QTL at chromosome 1 suggesting a major contribution of this enzyme to photosynthetic efficiency and thus WUE in potato. Indeed, Glutamine synthetase enzyme activity of leaf extracts was measured and found to be correlated with contrasting WUE phenotypes.
AB - Potato (Solanum tuberosum L.) closes its stomata at relatively low soil water deficits frequently encountered in normal field conditions resulting in unnecessary annual yield losses and extensive use of artificial irrigation. Therefore, unraveling the genetics underpinning variation in water use efficiency (WUE) of potato is important, but has been limited by technical difficulties in assessing the trait on individual plants and thus is poorly understood. In this study, a mapping population of potatoes has been robustly phenotyped, and considerable variation in WUE under well-watered conditions was observed. Two extreme WUE bulks of clones were identified and pools of genomic DNA from them as well as the parents were sequenced and mapped to reference potato genome. Following a novel data analysis approach, two highly resolved QTLs were found on chromosome 1 and 9. Interestingly, three genes encoding isoforms of cytosolic glutamine synthase were located in the QTL at chromosome 1 suggesting a major contribution of this enzyme to photosynthetic efficiency and thus WUE in potato. Indeed, Glutamine synthetase enzyme activity of leaf extracts was measured and found to be correlated with contrasting WUE phenotypes.
U2 - 10.1007/s00122-015-2573-2
DO - 10.1007/s00122-015-2573-2
M3 - Journal article
C2 - 26163769
SN - 0040-5752
VL - 128
SP - 2143
EP - 2153
JO - Theoretical and Applied Genetics
JF - Theoretical and Applied Genetics
IS - 11
ER -