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Cutibacterium acnes is an intracellular and intra-articular commensal of the human shoulder joint

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Cutibacterium acnes is an intracellular and intra-articular commensal of the human shoulder joint. / Hudek, Robert; Brobeil, Alexander; Brüggemann, Holger; Sommer, Frank; Gattenlöhner, Stefan; Gohlke, Frank.

I: Journal of Shoulder and Elbow Surgery, Bind 30, Nr. 1, 01.2021, s. 16-26.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Hudek, R, Brobeil, A, Brüggemann, H, Sommer, F, Gattenlöhner, S & Gohlke, F 2021, 'Cutibacterium acnes is an intracellular and intra-articular commensal of the human shoulder joint', Journal of Shoulder and Elbow Surgery, bind 30, nr. 1, s. 16-26. https://doi.org/10.1016/j.jse.2020.04.020

APA

Hudek, R., Brobeil, A., Brüggemann, H., Sommer, F., Gattenlöhner, S., & Gohlke, F. (2021). Cutibacterium acnes is an intracellular and intra-articular commensal of the human shoulder joint. Journal of Shoulder and Elbow Surgery, 30(1), 16-26. https://doi.org/10.1016/j.jse.2020.04.020

CBE

Hudek R, Brobeil A, Brüggemann H, Sommer F, Gattenlöhner S, Gohlke F. 2021. Cutibacterium acnes is an intracellular and intra-articular commensal of the human shoulder joint. Journal of Shoulder and Elbow Surgery. 30(1):16-26. https://doi.org/10.1016/j.jse.2020.04.020

MLA

Vancouver

Hudek R, Brobeil A, Brüggemann H, Sommer F, Gattenlöhner S, Gohlke F. Cutibacterium acnes is an intracellular and intra-articular commensal of the human shoulder joint. Journal of Shoulder and Elbow Surgery. 2021 jan;30(1):16-26. https://doi.org/10.1016/j.jse.2020.04.020

Author

Hudek, Robert ; Brobeil, Alexander ; Brüggemann, Holger ; Sommer, Frank ; Gattenlöhner, Stefan ; Gohlke, Frank. / Cutibacterium acnes is an intracellular and intra-articular commensal of the human shoulder joint. I: Journal of Shoulder and Elbow Surgery. 2021 ; Bind 30, Nr. 1. s. 16-26.

Bibtex

@article{d520b836f9df41469312b116778b0c52,
title = "Cutibacterium acnes is an intracellular and intra-articular commensal of the human shoulder joint",
abstract = "Background: Cutibacterium acnes (C acnes) is a mysterious member of the shoulder microbiome and is associated with chronic postoperative complications and low-grade infections. Nevertheless, it is unclear whether it represents a contaminant or whether it accounts for true infections. Because it can persist intracellularly in macrophages at several body sites, it might in fact be an intra-articular commensal of the shoulder joint.Methods: In 23 consecutive, otherwise healthy patients (17 male, 6 female; 58 years) who had no previous injections, multiple specimens were taken from the intra-articular tissue during first-time arthroscopic and open shoulder surgery. The samples were investigated by cultivation, genetic phylotyping, and immunohistochemistry using C acnes-specific antibodies and confocal laser scanning microscopy.Results: In 10 patients (43.5%), cultures were C acnes-positive. Phylotype IA1 dominated the subcutaneous samples (71%), whereas type II dominated the deep tissue samples (57%). Sixteen of 23 patients (69.6%) were C acnes-positive by immunohistochemistry; in total, 25 of 40 samples were positive (62.5%). Overall, 56.3% of glenohumeral immunohistochemical samples, 62.5% of subacromial samples, and 75% of acromioclavicular (AC) joint samples were positive. In 62.5% of the tested patients, C acnes was detected immunohistochemically to reside intracellularly within stromal cells and macrophages.Discussion: These data indicate that C acnes is a commensal of the human shoulder joint, where it persists within macrophages and stromal cells. Compared with culture-based methods, immunohistochemical staining can increase C acnes detection. Phylotype II seems to be most prevalent in the deep shoulder tissue. The high detection rate of C acnes in osteoarthritic AC joints might link its intra-articular presence to the initiation of osteoarthritis. (C) 2020 Journal of Shoulder and Elbow Surgery Board of Trustees. All rights reserved.",
keywords = "C acnes, Cross-Sectional Design, Cutibacterium acnes, Epidemiology Study, Level III, commensal, infection, intracellular, shoulder surgery, SURGERY, DIAGNOSIS, PROSTATE TISSUE, ARTHROPLASTY, IN-SITU HYBRIDIZATION, CULTURE, MODIC CHANGES, INFLAMMATION, PROPIONIBACTERIUM-ACNES, PERIPROSTHETIC INFECTIONS",
author = "Robert Hudek and Alexander Brobeil and Holger Br{\"u}ggemann and Frank Sommer and Stefan Gattenl{\"o}hner and Frank Gohlke",
note = "Copyright {\textcopyright} 2020 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Elsevier Inc. All rights reserved.",
year = "2021",
month = jan,
doi = "10.1016/j.jse.2020.04.020",
language = "English",
volume = "30",
pages = "16--26",
journal = "Journal of Shoulder and Elbow Surgery",
issn = "1058-2746",
publisher = "Mosby, Inc",
number = "1",

}

RIS

TY - JOUR

T1 - Cutibacterium acnes is an intracellular and intra-articular commensal of the human shoulder joint

AU - Hudek, Robert

AU - Brobeil, Alexander

AU - Brüggemann, Holger

AU - Sommer, Frank

AU - Gattenlöhner, Stefan

AU - Gohlke, Frank

N1 - Copyright © 2020 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Elsevier Inc. All rights reserved.

PY - 2021/1

Y1 - 2021/1

N2 - Background: Cutibacterium acnes (C acnes) is a mysterious member of the shoulder microbiome and is associated with chronic postoperative complications and low-grade infections. Nevertheless, it is unclear whether it represents a contaminant or whether it accounts for true infections. Because it can persist intracellularly in macrophages at several body sites, it might in fact be an intra-articular commensal of the shoulder joint.Methods: In 23 consecutive, otherwise healthy patients (17 male, 6 female; 58 years) who had no previous injections, multiple specimens were taken from the intra-articular tissue during first-time arthroscopic and open shoulder surgery. The samples were investigated by cultivation, genetic phylotyping, and immunohistochemistry using C acnes-specific antibodies and confocal laser scanning microscopy.Results: In 10 patients (43.5%), cultures were C acnes-positive. Phylotype IA1 dominated the subcutaneous samples (71%), whereas type II dominated the deep tissue samples (57%). Sixteen of 23 patients (69.6%) were C acnes-positive by immunohistochemistry; in total, 25 of 40 samples were positive (62.5%). Overall, 56.3% of glenohumeral immunohistochemical samples, 62.5% of subacromial samples, and 75% of acromioclavicular (AC) joint samples were positive. In 62.5% of the tested patients, C acnes was detected immunohistochemically to reside intracellularly within stromal cells and macrophages.Discussion: These data indicate that C acnes is a commensal of the human shoulder joint, where it persists within macrophages and stromal cells. Compared with culture-based methods, immunohistochemical staining can increase C acnes detection. Phylotype II seems to be most prevalent in the deep shoulder tissue. The high detection rate of C acnes in osteoarthritic AC joints might link its intra-articular presence to the initiation of osteoarthritis. (C) 2020 Journal of Shoulder and Elbow Surgery Board of Trustees. All rights reserved.

AB - Background: Cutibacterium acnes (C acnes) is a mysterious member of the shoulder microbiome and is associated with chronic postoperative complications and low-grade infections. Nevertheless, it is unclear whether it represents a contaminant or whether it accounts for true infections. Because it can persist intracellularly in macrophages at several body sites, it might in fact be an intra-articular commensal of the shoulder joint.Methods: In 23 consecutive, otherwise healthy patients (17 male, 6 female; 58 years) who had no previous injections, multiple specimens were taken from the intra-articular tissue during first-time arthroscopic and open shoulder surgery. The samples were investigated by cultivation, genetic phylotyping, and immunohistochemistry using C acnes-specific antibodies and confocal laser scanning microscopy.Results: In 10 patients (43.5%), cultures were C acnes-positive. Phylotype IA1 dominated the subcutaneous samples (71%), whereas type II dominated the deep tissue samples (57%). Sixteen of 23 patients (69.6%) were C acnes-positive by immunohistochemistry; in total, 25 of 40 samples were positive (62.5%). Overall, 56.3% of glenohumeral immunohistochemical samples, 62.5% of subacromial samples, and 75% of acromioclavicular (AC) joint samples were positive. In 62.5% of the tested patients, C acnes was detected immunohistochemically to reside intracellularly within stromal cells and macrophages.Discussion: These data indicate that C acnes is a commensal of the human shoulder joint, where it persists within macrophages and stromal cells. Compared with culture-based methods, immunohistochemical staining can increase C acnes detection. Phylotype II seems to be most prevalent in the deep shoulder tissue. The high detection rate of C acnes in osteoarthritic AC joints might link its intra-articular presence to the initiation of osteoarthritis. (C) 2020 Journal of Shoulder and Elbow Surgery Board of Trustees. All rights reserved.

KW - C acnes

KW - Cross-Sectional Design

KW - Cutibacterium acnes

KW - Epidemiology Study

KW - Level III

KW - commensal

KW - infection

KW - intracellular

KW - shoulder surgery

KW - SURGERY

KW - DIAGNOSIS

KW - PROSTATE TISSUE

KW - ARTHROPLASTY

KW - IN-SITU HYBRIDIZATION

KW - CULTURE

KW - MODIC CHANGES

KW - INFLAMMATION

KW - PROPIONIBACTERIUM-ACNES

KW - PERIPROSTHETIC INFECTIONS

U2 - 10.1016/j.jse.2020.04.020

DO - 10.1016/j.jse.2020.04.020

M3 - Journal article

C2 - 32741563

VL - 30

SP - 16

EP - 26

JO - Journal of Shoulder and Elbow Surgery

JF - Journal of Shoulder and Elbow Surgery

SN - 1058-2746

IS - 1

ER -