TY - JOUR
T1 - Culture media affect sex after IVF treatment—a detailed analysis of explanatory variables
AU - Guldager, T
AU - Gabrielsen, A
AU - Iversen, L H
AU - Kirkegaard, K
PY - 2024/5
Y1 - 2024/5
N2 - Purpose: The purpose of this study was to provide a detailed analysis of clinical and laboratory factors associated with skewed secondary sex ratio (SSR) after ART. Method: Retrospective cohort study of embryos resulting in live births, from frozen and fresh single blastocyst transfers. Embryos were cultured in either G-TL (n = 686) or Sage media (n = 685). Data was analyzed using a multivariate logistic regression model and a mixed model analysis. Results: Significantly more male singletons were born after culture in Sage media compared to G-TL media (odds ratio (OR) 1.34, 95% CI (1.05, 1.70), P = 0.02). Inner cell mass grade B vs A (OR 1.36 95% CI (1.05, 1.76), P = 0.02) and one previous embryo transfer (OR 1.49, 95% CI (1.03, 2.16), P = 0.03) were associated with a significantly higher probability of male child at birth. Factors associated with a reduced probability of male child were expansion grade 3 vs 5 (OR 0.66, 95% CI (10.45, 0.96), P = 0.03) and trophectoderm grade B vs A (OR 0.57, 95% CI (0.44, 0.74), P = 0.00). Male embryos developed significantly faster in Sage media compared to G-TL media for the stages of blastocyst (− 1.12 h, 95% CI (− 2.12, − 0.12)), expanded blastocyst (− 1.35 h, 95% CI (− 2.34, − 0.35)), and hatched blastocyst (− 1.75 h, 95% CI (− 2.99, − 0.52)). Conclusion: More male children were born after culture in Sage media compared to G-TL media. Male embryo development was affected by culture media. Our observations suggest that culture media impact male embryo quality selectively, thus potentially favoring the selection of male embryos.
AB - Purpose: The purpose of this study was to provide a detailed analysis of clinical and laboratory factors associated with skewed secondary sex ratio (SSR) after ART. Method: Retrospective cohort study of embryos resulting in live births, from frozen and fresh single blastocyst transfers. Embryos were cultured in either G-TL (n = 686) or Sage media (n = 685). Data was analyzed using a multivariate logistic regression model and a mixed model analysis. Results: Significantly more male singletons were born after culture in Sage media compared to G-TL media (odds ratio (OR) 1.34, 95% CI (1.05, 1.70), P = 0.02). Inner cell mass grade B vs A (OR 1.36 95% CI (1.05, 1.76), P = 0.02) and one previous embryo transfer (OR 1.49, 95% CI (1.03, 2.16), P = 0.03) were associated with a significantly higher probability of male child at birth. Factors associated with a reduced probability of male child were expansion grade 3 vs 5 (OR 0.66, 95% CI (10.45, 0.96), P = 0.03) and trophectoderm grade B vs A (OR 0.57, 95% CI (0.44, 0.74), P = 0.00). Male embryos developed significantly faster in Sage media compared to G-TL media for the stages of blastocyst (− 1.12 h, 95% CI (− 2.12, − 0.12)), expanded blastocyst (− 1.35 h, 95% CI (− 2.34, − 0.35)), and hatched blastocyst (− 1.75 h, 95% CI (− 2.99, − 0.52)). Conclusion: More male children were born after culture in Sage media compared to G-TL media. Male embryo development was affected by culture media. Our observations suggest that culture media impact male embryo quality selectively, thus potentially favoring the selection of male embryos.
KW - Culture media
KW - Embryo morphokinetics
KW - Expansion grade
KW - Secondary sex ratio
KW - Trophectoderm
UR - http://www.scopus.com/inward/record.url?scp=85187462508&partnerID=8YFLogxK
U2 - 10.1007/s10815-024-03081-1
DO - 10.1007/s10815-024-03081-1
M3 - Journal article
C2 - 38472564
AN - SCOPUS:85187462508
SN - 1058-0468
VL - 41
SP - 1181
EP - 1191
JO - Journal of Assisted Reproduction and Genetics
JF - Journal of Assisted Reproduction and Genetics
IS - 5
ER -