CT, PET and MR-Imaging in experimental baromedical research

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Introduction:
While pressurisation and decompression may provoke detrimental physiologic effects in humans and animals, many underlying
physiological phenomena remain unknown. Most studies on e.g. decompression sickness are conducted following pressurisation and decompression, in part because it is intrinsically difficult to study humans or animals inside a pressure chamber. We have developed a preclinical pressure chamber system compatible with CT, PET and MR-imaging during pressurisation up to 1.013 mPa, which allows for anatomical visualisations and measurements of certain physiological processes in vivo during pressurisation.

Material and methods:
Anaesthetised rats (simulated diving and control groups) underwent the following imaging protocols: First, a 3T clinical MRI-system was employed to evaluate in vivo cerebral relaxation parameters (T1, T2 and T2*). MRI was performed before, during 709 kPa pressurisation, and repeatedly after 500 kPa/min decompression. After MRI, venous bubble development was monitored using ultrasound. Second, preclinical μCT, PET/MRI, and high-field 9.4 T MR-Imaging systems evaluated changes in cerebral standard uptake value (SUV) of F-FDG, changes in cerebral blood flow (deltaCBF), T and T * relaxation, and blood brain barrier (BBB) breakage 90 min following decompression.

Results:
Simulated diving induced significant changes in all measured parameters during 3 T MRI. SUV of F-FDG was unchanged in both groups,
although following μCT-examination revealed intracranial bubbles. deltaCBF was significantly lower in dived vs. control rats, while T1-relaxation - but not T *-relaxation - was significantly elevated in both groups. No BBB-breakage was observed.

Conclusion: Our preliminary results obtained using CT-, PET- and MR-imaging of rats indicate that these non-invasive imaging techniques are sensitive to subtle cerebral changes during and following simulated diving. We believe that the described system is useful in many areas of experimental baromedical research.
OriginalsprogEngelsk
Udgivelsesår26 aug. 2017
StatusUdgivet - 26 aug. 2017
BegivenhedAnnual Meeting of the Scandinavian Physiological Society - Oslo, Norge
Varighed: 26 aug. 201628 aug. 2016

Konference

KonferenceAnnual Meeting of the Scandinavian Physiological Society
LandNorge
ByOslo
Periode26/08/201628/08/2016

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