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Correlative study of liquid in human bone by 3D neutron microscopy and lab-based X-ray μCT

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  • Maja Østergaard
  • Estrid Buhl Naver, Danmarks Tekniske Universitet
  • ,
  • Delia Schüpbach, Paul Scherrer Institute
  • ,
  • Anders Kaestner, Paul Scherrer Institute
  • ,
  • Markus Strobl, Paul Scherrer Institute, Københavns Universitet
  • ,
  • Annemarie Brüel
  • Jesper Skovhus Thomsen
  • Søren Schmidt, European Spallation Source ERIC
  • ,
  • Henning Friis Poulsen, Danmarks Tekniske Universitet
  • ,
  • Luise Theil Kuhn, Danmarks Tekniske Universitet
  • ,
  • Henrik Birkedal

Liquid plays an important role in bone that has a complex 3D hierarchical pore structure. However, liquid (water) is difficult to discern from e.g. an organic matrix by X-ray imaging. Therefore, we use a correlative approach using both high resolution X-ray and neutron imaging. Human femoral bone with liquid adsorbed into some of the pores was imaged with both the Neutron Microscope at the ICON beamline, SINQ at PSI, and by lab-based μCT using 2.7 μm voxel size. Segmentation of the two datasets showed that, even though the liquid was clearly distinguishable in the neutron data and not in the X-ray data, it remained challenging to segment it from bone due to overlaps of peaks in the gray level histograms. In consequence, segmentations from X-ray and neutron data varied significantly. To address this issue, the segmented X-ray porosities was overlaid on the neutron data, making it possible to localize the liquid in the vascular porosities of the bone sample and use the neutron attenuation to identify it as H2O. The contrast in the neutron images was lowered slightly between the bone and the liquid compared to the bone and the air. This correlative study shows that the complementary use of X-rays and neutrons is very favorable, since H2O is very distinct in the neutron data, while D2O, H2O, and organic matter can barely be distinguished from air in the X-ray data.

Antal sider9
StatusUdgivet - okt. 2023

Bibliografisk note

Funding Information:
We acknowledge support from the ESS lighthouse on hard materials in 3D, SOLID, funded by the Danish Agency for Science and Higher Education, grant number 8144-00002B. We thank the Danish Agency for Science, Technology, and Innovation for funding the instrument center DanScatt. Use of the Novo Nordisk Foundation research infrastructure AXIA (grant NNF19OC0055801) and support from Dr. Nina K. Wittig is gratefully acknowledged. Technical support in sample preparation by Dr. Carsten Pedersen is gratefully acknowledged. This work is based on experiments performed at the Swiss spallation neutron source SINQ, Paul Scherrer Institute, Villigen, Switzerland.

Publisher Copyright:
© 2023 The Author(s)

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