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Co-occurrence of organic and inorganic N influences asparagine uptake and amino acid profiles in white clover

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Background: Direct plant uptake of organic nitrogen (N) may be important for plant N nutrition, but we lack knowledge of how the concentration and form of external N influence organic N uptake and plant N status. Aims: We investigated the uptake of the amino acid asparagine (Asn) in white clover in the presence of different inorganic, organic and total N concentrations. Methods: Actively N2-fixing white clover seedlings were for one week exposed to combinations of (Formula presented.) (3–30 µmol N kg−1 sand DW) and Asn (3–30 µmol N kg−1 sand DW), whereafter the Asn uptake rate was determined by addition of 13C4-Asn. Shoot and root amino acid profiles were also analyzed. Results: Increasing (Formula presented.) and total N concentrations decreased 13C4-Asn uptake rates and internal clover Asn content. In addition, total N and (Formula presented.) also affected amino acid profiles, with Asn, Asp, Glu, Gln, Cys, Gly, Pro, Ser, and Ala being more related to the low N doses, and Thr, Val, Ile, Leu, Phe, Tyr, Trp, and Met being more abundant at increasing N doses. Conclusions: Asn uptake rate in white clover is reduced by increasing inorganic N. Plant amino acid profiles are likely to be a more sensitive indicator of N supply.

OriginalsprogEngelsk
TidsskriftJournal of Plant Nutrition and Soil Science
Vol/bind184
Nummer1
Sider (fra-til)162-172
Antal sider11
ISSN1436-8730
DOI
StatusUdgivet - feb. 2021

Bibliografisk note

Funding Information:
The study was financially supported by The Independent Research Fund Denmark ? Technology and Production (Project no. 1335?00760B). The authors would like to thank Professor Wanda Ma?ek (Maria Curie Sk?odowska University in Lublin, Poland) for providing bacterial strain Rhizobium leguminosarum bv. trifolii TA1 for the plant inoculation.

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© 2020 Wiley-VCH GmbH

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Copyright 2021 Elsevier B.V., All rights reserved.

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