Controlling Styrene Maleic Acid Lipid Particles through RAFT

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

DOI

  • Anton A. A. Smith
  • Henriette E. Autzen
  • Tomas Laursen, Univ Copenhagen, University of Copenhagen, Dept Plant & Environm Sci PLEN, Lawrence Berkeley National Laboratory, CA
  • ,
  • Vincent Wu, Univ Calif Berkeley, University of California Berkeley, University of California System, Chem Mat Sci & Engn
  • ,
  • Max Yen, Univ Calif Berkeley, University of California Berkeley, University of California System, Chem Mat Sci & Engn
  • ,
  • Aaron Hall, Univ Calif Berkeley, University of California Berkeley, University of California System, Chem Mat Sci & Engn
  • ,
  • Scott D. Hansen, Univ Calif Berkeley, University of California Berkeley, University of California System, Calif Inst Quantitat Biosci QB3
  • ,
  • Yifan Cheng, UCSF, University of California San Francisco, University of California System, Biochem & Biophys
  • ,
  • Ting Xu, Univ Calif Berkeley, University of California Berkeley, University of California System, Chem Mat Sci & Engn

The ability of styrene maleic acid copolymers to dissolve lipid membranes into nanosized lipid particles is a facile method of obtaining membrane proteins in solubilized lipid discs while conserving part of their native lipid environment. While the currently used copolymers can readily extract membrane proteins in native nanodiscs, their highly disperse composition is likely to influence the dispersity of the discs as well as the extraction efficiency. In this study, reversible addition-fragmentation chain transfer was used to control the polymer architecture and dispersity of molecular weights with a high-precision. Based on Monte Carlo simulations of the polymerizations, the monomer composition was predicted and allowed a structure function analysis of the polymer architecture, in relation to their ability to assemble into lipid nanoparticles. We show that a higher degree of control of the polymer architecture generates more homogeneous samples. We hypothesize that low dispersity copolymers, with control of polymer architecture are an ideal framework for the rational design of polymers for customized isolation and characterization of integral membrane proteins in native lipid bilayer systems.

OriginalsprogEngelsk
TidsskriftBiomacromolecules
Vol/bind18
Nummer11
Sider (fra-til)3706-3713
Antal sider8
ISSN1525-7797
DOI
StatusUdgivet - nov. 2017
Begivenhed4th Symposium on Innovative Polymers for Controlled Delivery (SIPCD) - Suzhou
Varighed: 23 sep. 201626 sep. 2016

Konference

Konference4th Symposium on Innovative Polymers for Controlled Delivery (SIPCD)
BySuzhou
Periode23/09/201626/09/2016

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