Control of enzyme reactions by a reconfigurable DNA nanovault

TY - JOUR

T1 - Control of enzyme reactions by a reconfigurable DNA nanovault

AU - Grossi, Guido

AU - Jepsen, Mette Dalgaard Ebbesen

AU - Kjems, Jorgen

AU - Andersen, Ebbe Sloth

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Biological systems use compartmentalisation as a general strategy to control enzymatic reactions by precisely regulating enzyme-substrate interactions. With the advent of DNA nanotechnology, it has become possible to rationally design DNA-based nano-containers with programmable structural and dynamic properties. These DNA nanostructures have been used to cage enzymes, but control over enzyme-substrate interactions using a dynamic DNA nanostructure has not been achieved yet. Here we introduce a DNA origami device that functions as a nanoscale vault: an enzyme is loaded in an isolated cavity and the access to free substrate molecules is controlled by a multi-lock mechanism. The DNA vault is characterised for features such as reversible opening/closing, cargo loading and wall porosity, and is shown to control the enzymatic reaction catalysed by an encapsulated protease. The DNA vault represents a general concept to control enzyme-substrate interactions by inducing conformational changes in a rationally designed DNA nanodevice.

AB - Biological systems use compartmentalisation as a general strategy to control enzymatic reactions by precisely regulating enzyme-substrate interactions. With the advent of DNA nanotechnology, it has become possible to rationally design DNA-based nano-containers with programmable structural and dynamic properties. These DNA nanostructures have been used to cage enzymes, but control over enzyme-substrate interactions using a dynamic DNA nanostructure has not been achieved yet. Here we introduce a DNA origami device that functions as a nanoscale vault: an enzyme is loaded in an isolated cavity and the access to free substrate molecules is controlled by a multi-lock mechanism. The DNA vault is characterised for features such as reversible opening/closing, cargo loading and wall porosity, and is shown to control the enzymatic reaction catalysed by an encapsulated protease. The DNA vault represents a general concept to control enzyme-substrate interactions by inducing conformational changes in a rationally designed DNA nanodevice.

KW - STRAND-DISPLACEMENT

KW - ORIGAMI

KW - PROTEIN

KW - ENCAPSULATION

KW - CONSTRUCTION

KW - NANOREACTOR

KW - SHAPES

KW - COMPARTMENTALIZATION

KW - NANOSTRUCTURES

KW - TETRAHEDRON

UR - http://www.scopus.com/inward/record.url?scp=85031924847&partnerID=8YFLogxK

U2 - 10.1038/s41467-017-01072-8

DO - 10.1038/s41467-017-01072-8

M3 - Journal article

C2 - 29051565

SN - 2041-1723

VL - 8

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 992

ER -