@inbook{df325f655ba94e58866341233fcde3a3,
title = "Construction of Small Genes with Repetitive Elements Using Oligo Extension and Golden Gate Assembly",
abstract = "Gene synthesis efficiency has greatly improved in recent years but is limited when it comes to repetitive sequences and results in synthesis failure or delays by DNA synthesis vendors. Here, we describe a method for the assembly of small synthetic genes with repetitive elements: First, a gene of interest is split in silico into small synthons of up to 80 base pairs flanked by Golden Gate-compatible overhangs. Then synthons are made by oligo extension and finally assembled into a synthetic gene by Golden Gate assembly.",
keywords = "Gene synthesis, Golden Gate cloning, Molecular cloning, Repetitive DNA sequences, Synthetic biology",
author = "Nguyen, \{Michael T.A.\} and Andersen, \{Ebbe Sloth\} and Georgios Pothoulakis",
year = "2025",
doi = "10.1007/978-1-0716-4220-7\_12",
language = "English",
isbn = "978-1-0716-4094-4",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "219--227",
editor = "Daniel Schindler",
booktitle = "Golden Gate Cloning",
}