Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment

Mikkel Andreas Glaring; Jan Kjolhede Vester; Peter Stougaard

Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment

Mikkel Andreas Glaring, Jan Kjolhede Vester, Peter Stougaard

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Abstract

Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a source of bacteria and enzymes adapted to these conditions. They have also highlighted the limitations of cultivation-based methods in this extreme environment and metagenomic approaches may provide access to novel extremophilic enzymes from the uncultured majority of bacteria. Here, we describe the construction and screening of a metagenomic library of the prokaryotic community inhabiting the ikaite columns.

Originalsprog	Engelsk
Tidsskrift	Methods in Molecular Biology
Vol/bind	1539
Sider (fra-til)	43-55
Antal sider	12
ISSN	1064-3745
Status	Udgivet - 2017
Udgivet eksternt	Ja

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title = "Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment",

abstract = "Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a source of bacteria and enzymes adapted to these conditions. They have also highlighted the limitations of cultivation-based methods in this extreme environment and metagenomic approaches may provide access to novel extremophilic enzymes from the uncultured majority of bacteria. Here, we describe the construction and screening of a metagenomic library of the prokaryotic community inhabiting the ikaite columns.",

keywords = "Biotechnology, Cell extraction, Extreme environments, Extremophilic enzymes, Functionbased screen, Metagenome",

author = "Glaring, {Mikkel Andreas} and Vester, {Jan Kjolhede} and Peter Stougaard",

year = "2017",

language = "English",

volume = "1539",

pages = "43--55",

journal = "Methods in Molecular Biology",

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TY - JOUR

T1 - Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment

AU - Glaring, Mikkel Andreas

AU - Vester, Jan Kjolhede

AU - Stougaard, Peter

PY - 2017

Y1 - 2017

N2 - Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a source of bacteria and enzymes adapted to these conditions. They have also highlighted the limitations of cultivation-based methods in this extreme environment and metagenomic approaches may provide access to novel extremophilic enzymes from the uncultured majority of bacteria. Here, we describe the construction and screening of a metagenomic library of the prokaryotic community inhabiting the ikaite columns.

AB - Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a source of bacteria and enzymes adapted to these conditions. They have also highlighted the limitations of cultivation-based methods in this extreme environment and metagenomic approaches may provide access to novel extremophilic enzymes from the uncultured majority of bacteria. Here, we describe the construction and screening of a metagenomic library of the prokaryotic community inhabiting the ikaite columns.

KW - Biotechnology

KW - Cell extraction

KW - Extreme environments

KW - Extremophilic enzymes

KW - Functionbased screen

KW - Metagenome

UR - http://www.scopus.com/inward/record.url?scp=85002213746&partnerID=8YFLogxK

M3 - Journal article

SN - 1064-3745

VL - 1539

SP - 43

EP - 55

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment

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