Constitutive expression of Asparaginase in Gossypium hirsutum triggers insecticidal activity against Bemisia tabaci

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  • Ambreen Gul, University of the Punjab
  • ,
  • Ghulam Hussain, University of the Punjab
  • ,
  • Adnan Iqbal, University of the Punjab
  • ,
  • Abdul Qayyum Rao, University of the Punjab
  • ,
  • Salah ud Din, University of the Punjab
  • ,
  • Aneela Yasmeen, University of the Punjab
  • ,
  • Naila Shahid, University of the Punjab
  • ,
  • Ammara Ahad, University of the Punjab
  • ,
  • Ayesha Latif, University of the Punjab
  • ,
  • Saira Azam, University of the Punjab
  • ,
  • Tahir Rehman Samiullah, University of the Punjab
  • ,
  • Samina Hassan, University of the Punjab, Kinnaird College for Women
  • ,
  • Ahmad Ali Shahid, University of the Punjab
  • ,
  • Tayyab Husnain, University of the Punjab

Whitefly infestation of cotton crop imparts enormous damage to cotton yield by severely affecting plant health, vigour and transmitting Cotton Leaf Curl Virus (CLCuV). Genetic modification of cotton helps to overcome both the direct whitefly infestation as well as CLCuV based cotton yield losses. We have constitutively overexpressed asparaginase (ZmASN) gene in Gossypium hirsutum to overcome the cotton yield losses imparted by whitefly infestation. We achieved 2.54% transformation efficiency in CIM-482 by Agrobacterium-mediated shoot apex transformation method. The relative qRT-PCR revealed 40-fold higher transcripts of asparaginase in transgenic cotton line vs. non-transgenic cotton lines. Metabolic analysis showed higher contents of aspartic acid and glutamic acid in seeds and phloem sap of the transgenic cotton lines. Phenotypically, the transgenic cotton lines showed vigorous growth and height, greater number of bolls, and yield. Among six representative transgenic cotton lines, line 14 had higher photosynthetic rate, stomatal conductance, smooth fiber surface, increased fiber convolutions (SEM analysis) and 95% whitefly mortality as compared to non-transgenic cotton line. The gene integration analysis by fluorescence in situ hybridization showed single copy gene integration at chromosome number 1. Collectively, asparaginase gene demonstrated potential to control whitefly infestation, post-infestation damages and improve cotton plant health and yield: a pre-requisite for farmer’s community.

OriginalsprogEngelsk
Artikelnummer8958
TidsskriftScientific Reports
Vol/bind10
Nummer1
Antal sider11
ISSN2045-2322
DOI
StatusUdgivet - 2020

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