Conjunctival intraepithelial neoplasia and carcinoma: Distinct clinical and histological features in relation to human papilloma virus status

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Ingvild Ramberg, Københavns Universitet
  • ,
  • Peter Bjerre Toft, Københavns Universitet
  • ,
  • Jeanette Bæhr Georgsen
  • ,
  • Volkert DIrk Siersma, Københavns Universitet
  • ,
  • Mikkel Funding
  • David Hebbelstrup Jensen, Københavns Universitet
  • ,
  • Christian Von Buchwald, Københavns Universitet
  • ,
  • Steffen Heegaard, Københavns Universitet

Aims Human papillomavirus (HPV) is considered a causative agent for the development of a broad range of human carcinomas. The role of HPV in the development of conjunctival intraepithelial neoplasia (CIN) and carcinoma (cSCC) remains unclear. The purpose of the present study was to investigate the HPV prevalence in a nationwide cohort and to describe clinical and histopathological features in relation to HPV status. Methods All cases of CIN and cSCC in Denmark from 1980 to 2016 were included. We combined p16 immunohistochemistry (IHC), RNA in situ hybridisation (RNA ISH) and HPV DNA PCR to detect HPV. The results were correlated to clinical and histopathological parameters. Results One hundred twelve primary tumours and 33 recurrent tumours were included for HPV analysis. Twenty-four (21%) of the primary tumours were HPV positive by PCR. Eighteen of out 19 HPV-positive tumours were positive by RNA ISH. HPV16 was the most prevalent genotype (n=18, 75%). The patients with HPV-positive tumours were significantly younger (mean difference 11.5 years, 95% CI 5.2 to 17.9, p=0.0005) and had a higher recurrence compared with patients with HPV-negative tumours (HR 2.30, 95% CI 1.02 to 5.21, p=0.046). The HPV-positive tumours were associated with a positive p16 IHC and a non-keratinising morphology. Conclusion We describe distinct clinical and histopathological features associated with HPV status in cSCC. The finding of transcriptionally active HPV in this material lends support to a causal role of HPV in a subset of cSCC.

TidsskriftBritish Journal of Ophthalmology
Sider (fra-til)878-883
Antal sider6
StatusUdgivet - 1 jun. 2021

Bibliografisk note

Funding Information:
or the acquisition, analysis or interpretation of data for the work: IR, PBT, JBG, VDS, MF, DHJ, CvB, SH. Drafting the work or revising it critically for important intellectual content: IR, PBT, JBG, VDS, MF, DHJ, CvB, SH. Final approval of the version to be published: IR, PBT, JBG, VDS, MF, DHJ, CvB, SH. Agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved: IR, PBT, JBG, VDS, MF, DHJ, CvB, SH. Funding This work was supported by Arvid Schrøder and Ketty Lydia Larsen Schrøder Foundation, the Synoptik Foundation, ’Fight for Sight, Denmark’, Faculty of Health and Medical Science, Copenhagen University and the Danish Eye Research Foundation.

Publisher Copyright:
© Author(s) (or their employer(s)) 2021. No commercial re-use. See rights and permissions. Published by BMJ.

Copyright 2021 Elsevier B.V., All rights reserved.

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