Claw disorders are a major problem for health, welfare, and economy in dairy production. This retrospective observational study investigated the association between cow-level prevalence of claw horn disruptive lesions and two different trimming methods - the traditional Danish Method (DAM) and the White Line Atlas Method (WLAM). Trimming records from four herds in Denmark over a 4-year period were analysed. Within each herd, claw trimming was performed with the DAM for the first 2 years, and the WLAM for the next 2 years. The data comprised 3316 claw trimmings of 1027 cows with the WLAM and 3898 claw trimmings of 1080 cows with the DAM. The association between claw trimming method and claw lesions was determined using binominal logistic analysis. There were significant differences between trimming methods for sole haemorrhage (odds ratio = 0.44), sole ulcer (odds ratio = 0.42), and white line separation (odds ratio = 0.64), with a lower prevalence during the period of trimming with the WLAM. No significant difference between trimming methods could be found in the occurrence of white line abscess or double sole. The lower prevalence of claw horn disruption lesions found in this study when cows were trimmed with WLAM justifies and necessitates further experimental studies of claw trimming methods to validate these findings.