Comparing Earthworm Biodiversity Estimated by DNA Metabarcoding and Morphology-based Approaches

Mille Anna Lilja; Zivile Buivydaite; Athanasios Zervas; Paul Henning Krogh; Benni Winding Winding; Anne Winding; Rumakanta Sapkota

doi:10.1016/j.apsoil.2022.104798

Comparing Earthworm Biodiversity Estimated by DNA Metabarcoding and Morphology-based Approaches

Mille Anna Lilja, Zivile Buivydaite, Athanasios Zervas, Paul Henning Krogh, Benni Winding Winding, Anne Winding, Rumakanta Sapkota^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

3 Citationer (Scopus)

7 Downloads (Pure)

Abstract

Conventional identification based on morphology is today's tool of choice for monitoring earthworm diversity. However, it requires skills to distinguish specific features, which can become the bottleneck in soil ecological studies. DNA-based metabarcoding is increasingly used to monitor diversity of soil organisms. Few studies have compared this method with conventional methods. The aim of the study was to compare metabarcoding based on the mitochondrial 16S rRNA region with conventional morphology-based identification, where metabarcoding captured more species (eight) than identified by conventional method (five). Additionally, we tested the effects of two commercial DNA extraction kits: PowerSoil based on 0.25 g of soil and PowerMax based on 10 g of soil (both from Qiagen), where the PowerMax covered higher richness than PowerSoil.

Originalsprog	Engelsk
Artikelnummer	104798
Tidsskrift	Applied Soil Ecology
Vol/bind	185
Antal sider	4
ISSN	0929-1393
DOI	https://doi.org/10.1016/j.apsoil.2022.104798
Status	Udgivet - maj 2023

Adgang til dokumentet

10.1016/j.apsoil.2022.104798Licens: CC BY

1-s2.0-S0929139322004140-mainForlagets udgivne version, 544 KBLicens: CC BY

1 Igangværende
2 Afsluttet

Rådgivning om jordbundsinvertebrater og deres økologi, diversitet og økotoksikologi
Krogh, P. H. (PI)
01/01/1997 → …
Projekter: Projekt › Rådgivende projekt
eDNA Center - Applied molecular tools for environmental monitoring and research
Winding, A. (PI), Sapkota, R. (PI), Zervas, T. (PI), Hendriksen, N. B. (PI), Stougaard, P. (PI), Thøgersen, M. S. (PI), Barbosa Anesio, A. M. (PI), Zeng, Y. (PI), Johansen, A. (PI), Jacobsen, C. S. (PI), Ørby, P. V. (PI), Stæhr, P. A. (PI), Hansen, J. L. S. (PI), Jakobsen, H. (PI), Krause-Jensen, D. (PI), Krogh, P. H. (PI), Schmidt, N. M. (PI), Scheel, M. (PI), Andersen, L. W. (PI), Nicolaisen, M. (PI), Madsen, M. V. (PI), Santos, S. (PI), Kudjordjie, E. N. (PI) & Sikder, M. M. (PI)
01/01/2020 → 30/11/2023
Projekter: Projekt › Forskning
Klima- og miljøeffekter af nitrifikationshæmmere
Winding, A. (Projektkoordinator), Krogh, P. H. (PI), Bruun, S. (PI), Petersen, S. O. (PI), Sapkota, R. (PI), Buivydaite, Z. (PI), Thane, T. (PI) & Bruus, M. (PI)
01/09/2019 → 28/02/2024
Projekter: Projekt › Forskning

Citationsformater

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title = "Comparing Earthworm Biodiversity Estimated by DNA Metabarcoding and Morphology-based Approaches",

abstract = "Conventional identification based on morphology is today's tool of choice for monitoring earthworm diversity. However, it requires skills to distinguish specific features, which can become the bottleneck in soil ecological studies. DNA-based metabarcoding is increasingly used to monitor diversity of soil organisms. Few studies have compared this method with conventional methods. The aim of the study was to compare metabarcoding based on the mitochondrial 16S rRNA region with conventional morphology-based identification, where metabarcoding captured more species (eight) than identified by conventional method (five). Additionally, we tested the effects of two commercial DNA extraction kits: PowerSoil based on 0.25 g of soil and PowerMax based on 10 g of soil (both from Qiagen), where the PowerMax covered higher richness than PowerSoil.",

keywords = "Community barcoding, DNA extraction, Environmental DNA, Mitochondrial 16S rRNA, Soil",

author = "Lilja, {Mille Anna} and Zivile Buivydaite and Athanasios Zervas and Krogh, {Paul Henning} and Winding, {Benni Winding} and Anne Winding and Rumakanta Sapkota",

year = "2023",

month = may,

doi = "10.1016/j.apsoil.2022.104798",

language = "English",

volume = "185",

journal = "Applied Soil Ecology",

issn = "0929-1393",

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T1 - Comparing Earthworm Biodiversity Estimated by DNA Metabarcoding and Morphology-based Approaches

AU - Lilja, Mille Anna

AU - Buivydaite, Zivile

AU - Zervas, Athanasios

AU - Krogh, Paul Henning

AU - Winding, Benni Winding

AU - Winding, Anne

AU - Sapkota, Rumakanta

PY - 2023/5

Y1 - 2023/5

N2 - Conventional identification based on morphology is today's tool of choice for monitoring earthworm diversity. However, it requires skills to distinguish specific features, which can become the bottleneck in soil ecological studies. DNA-based metabarcoding is increasingly used to monitor diversity of soil organisms. Few studies have compared this method with conventional methods. The aim of the study was to compare metabarcoding based on the mitochondrial 16S rRNA region with conventional morphology-based identification, where metabarcoding captured more species (eight) than identified by conventional method (five). Additionally, we tested the effects of two commercial DNA extraction kits: PowerSoil based on 0.25 g of soil and PowerMax based on 10 g of soil (both from Qiagen), where the PowerMax covered higher richness than PowerSoil.

AB - Conventional identification based on morphology is today's tool of choice for monitoring earthworm diversity. However, it requires skills to distinguish specific features, which can become the bottleneck in soil ecological studies. DNA-based metabarcoding is increasingly used to monitor diversity of soil organisms. Few studies have compared this method with conventional methods. The aim of the study was to compare metabarcoding based on the mitochondrial 16S rRNA region with conventional morphology-based identification, where metabarcoding captured more species (eight) than identified by conventional method (five). Additionally, we tested the effects of two commercial DNA extraction kits: PowerSoil based on 0.25 g of soil and PowerMax based on 10 g of soil (both from Qiagen), where the PowerMax covered higher richness than PowerSoil.

KW - Community barcoding

KW - DNA extraction

KW - Environmental DNA

KW - Mitochondrial 16S rRNA

KW - Soil

U2 - 10.1016/j.apsoil.2022.104798

DO - 10.1016/j.apsoil.2022.104798

M3 - Journal article

SN - 0929-1393

VL - 185

JO - Applied Soil Ecology

JF - Applied Soil Ecology

M1 - 104798

ER -

Comparing Earthworm Biodiversity Estimated by DNA Metabarcoding and Morphology-based Approaches

Abstract

Adgang til dokumentet

Fingeraftryk

Projekter

Rådgivning om jordbundsinvertebrater og deres økologi, diversitet og økotoksikologi

eDNA Center - Applied molecular tools for environmental monitoring and research

Klima- og miljøeffekter af nitrifikationshæmmere

Citationsformater