Comparative Genomics, Pangenome, and Phylogenomic Analyses of Brenneria spp., and Delineation of Brenneria izadpanahii sp. nov.

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DOI

  • Meysam Bakhshi ganje, Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Iran
  • John Mackay, University of Oxford, Department of Plant Sciences, Storbritannien
  • Mogens Nicolaisen
  • Masoud Shams-Bakhsh, Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Iran

Brenneria species are bacterial plant pathogens mainly affecting woody plants. Association of all members with devastating disorders (e.g., acute oak decline in Iran and United Kingdom) are due to adaptation and pathogenic behavior in response to host and environmental factors. Some species, including B. goodwinii, B. salicis, and B. nigrifluens, also show endophytic residence. Here we show that all species including novel Brenneria sp. are closely related. Gene-based and genome/pangenomebased phylogeny divide the genus into two distinct lineages, Brenneria clades A and B. The two clades were functionally distinct and were consistent with their common and special potential activities as determined via annotation of functional domains. Pangenome analysis demonstrated that the core pathogenicity factors were highly conserved, an hrp gene cluster encoding a type III secretion system was found in all species except B. corticis. An extensive repertoire of candidate virulence factors was identified. Comparative genomics indicated a repertoire of plant cell wall degrading enzymes, metabolites/antibiotics, and numerous prophages providing new insights into Brenneria-host interactions and appropriate targets for further characterization. This work not only documented the genetic differentiation of Brenneria species but also delineates a more functionally driven understanding of Brenneria by comparison with relevant Pectobacteriaceae thereby substantially enriching the extent of information available for functional genomic investigations.

OriginalsprogEngelsk
TidsskriftPhytopathology
Vol/bind111
Nummer1
Sider (fra-til)78-95
Antal sider18
ISSN0031-949X
DOI
StatusUdgivet - jan. 2021

    Forskningsområder

  • Brenneria izadpanahii, Brenneria spp., comparative genomics, pangenome analysis, phylogenomics

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