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Combining pressing and alkaline extraction to increase protein yield from Ulva fenestrata biomass

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Many seaweed species have a high production potential and attract interest as future protein sources. A high fiber and ash content, however, demand extraction of the protein to improve its digestibility and protein utilization in food or feed. This study explores three different approaches for protein extraction from Ulva fenestrata in order to maximize the protein extraction yield. Soluble protein was recovered either by mechanical pressing or by homogenization and osmotic shock of the biomass followed by alkaline extraction. The soluble protein was then concentrated by isoelectric precipitation. A combined procedure was carried out by pressing the biomass and following subjecting the residual pulp fraction to homogenization, osmotic shock and alkaline extraction. The three methods were ranked as follows with respect to protein extraction yield (as % of biomass protein); the combined method (23.9 ± 0.3%)> the alkaline extraction (6.8 ± 0.2%)> mechanical pressing (5.0 ± 0.2%). The significant increase when combining the methods was ascribed to a high precipitation yield after alkaline extraction of the pulp, hypothesized to be due to a reduced conductivity of the alkali-soluble protein fraction when derived from pulp rather than whole biomass.

TidsskriftFood and Bioproducts Processing
Sider (fra-til)80-85
Antal sider6
StatusUdgivet - jul. 2022

Bibliografisk note

Funding Information:
The study was primarily funded by Green Development and Demonstration Programme (GUDP), Denmark , j. no 34009–19-1615 and Center for Circular Bioeconomy (CBIO) at Aarhus University, Denmark. The study also received support by the Swedish Foundation for Strategic Research (project no. 2820005 ) and Formas, Sweden (project 2018–01839 ).

Publisher Copyright:
© 2022 The Author(s)

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