Colostrum protein uptake in neonatal lambs examined by descriptive and quantitative liquid chromatography-tandem mass spectrometry

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Colostrum protein uptake in neonatal lambs examined by descriptive and quantitative liquid chromatography-tandem mass spectrometry. / Hernandez-Castellano, Lorenzo E; Argueello, Anastasio; Almeida, Andre M; Castro, Noemi; Bendixen, Emøke.

I: Journal of Dairy Science, Bind 98, Nr. 1, 01.2015, s. 135-147.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Hernandez-Castellano, LE, Argueello, A, Almeida, AM, Castro, N & Bendixen, E 2015, 'Colostrum protein uptake in neonatal lambs examined by descriptive and quantitative liquid chromatography-tandem mass spectrometry', Journal of Dairy Science, bind 98, nr. 1, s. 135-147. https://doi.org/10.3168/jds.2014-8143

APA

Hernandez-Castellano, L. E., Argueello, A., Almeida, A. M., Castro, N., & Bendixen, E. (2015). Colostrum protein uptake in neonatal lambs examined by descriptive and quantitative liquid chromatography-tandem mass spectrometry. Journal of Dairy Science, 98(1), 135-147. https://doi.org/10.3168/jds.2014-8143

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MLA

Vancouver

Author

Hernandez-Castellano, Lorenzo E ; Argueello, Anastasio ; Almeida, Andre M ; Castro, Noemi ; Bendixen, Emøke. / Colostrum protein uptake in neonatal lambs examined by descriptive and quantitative liquid chromatography-tandem mass spectrometry. I: Journal of Dairy Science. 2015 ; Bind 98, Nr. 1. s. 135-147.

Bibtex

@article{2eb2b8fb0d2d41898f78b25ff08711eb,
title = "Colostrum protein uptake in neonatal lambs examined by descriptive and quantitative liquid chromatography-tandem mass spectrometry",
abstract = "Colostrum intake is a key factor for newborn ruminant survival because the placenta does not allow the transfer of immune components. Therefore, newborn ruminants depend entirely on passive immunity transfer from the mother to the neonate, through the suckling of colostrum. Understanding the importance of specific colostrum proteins has gained significant attention in recent years. However, proteomics studies of sheep colostrum and their uptake in neonate lambs has not yet been presented. The aim of this study was to describe the proteomes of sheep colostrum and lamb blood plasma, using sodium dodecyl sulfate-PAGE for protein separation and in-gel digestion, followed by liquid chromatography-tandem mass spectrometry of resulting tryptic peptides for protein identification. An isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomics approach was subsequently used to provide relative quantification of how neonatal plasma protein concentrations change as an effect of colostrum intake. The results of this study describe the presence of 70 proteins in the ovine colostrum proteome. Furthermore, colostrum intake resulted in an increase of 8 proteins with important immune functions in the blood plasma of lambs. Further proteomic studies will be necessary, particularly using the selected reaction monitoring approach, to describe in detail the role of specific colostrum proteins for immune transfer to the neonate.",
keywords = "colostrum, immunity, proteomics, isobaric tag for relative and absolute quantitation (iTRAQ), PEPTIDOGLYCAN RECOGNITION PROTEINS, INNATE IMMUNE-RESPONSE, LOW-ABUNDANCE PROTEINS, BOVINE MAMMARY-GLAND, MAJORERA GOAT KIDS, IMMUNOGLOBULIN-G, A-IV, PASSIVE TRANSFER, SHORT-COMMUNICATION, TRYPSIN-INHIBITOR",
author = "Hernandez-Castellano, {Lorenzo E} and Anastasio Argueello and Almeida, {Andre M} and Noemi Castro and Em{\o}ke Bendixen",
year = "2015",
month = jan,
doi = "10.3168/jds.2014-8143",
language = "English",
volume = "98",
pages = "135--147",
journal = "Journal of Dairy Science",
issn = "0022-0302",
publisher = "Elsevier Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Colostrum protein uptake in neonatal lambs examined by descriptive and quantitative liquid chromatography-tandem mass spectrometry

AU - Hernandez-Castellano, Lorenzo E

AU - Argueello, Anastasio

AU - Almeida, Andre M

AU - Castro, Noemi

AU - Bendixen, Emøke

PY - 2015/1

Y1 - 2015/1

N2 - Colostrum intake is a key factor for newborn ruminant survival because the placenta does not allow the transfer of immune components. Therefore, newborn ruminants depend entirely on passive immunity transfer from the mother to the neonate, through the suckling of colostrum. Understanding the importance of specific colostrum proteins has gained significant attention in recent years. However, proteomics studies of sheep colostrum and their uptake in neonate lambs has not yet been presented. The aim of this study was to describe the proteomes of sheep colostrum and lamb blood plasma, using sodium dodecyl sulfate-PAGE for protein separation and in-gel digestion, followed by liquid chromatography-tandem mass spectrometry of resulting tryptic peptides for protein identification. An isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomics approach was subsequently used to provide relative quantification of how neonatal plasma protein concentrations change as an effect of colostrum intake. The results of this study describe the presence of 70 proteins in the ovine colostrum proteome. Furthermore, colostrum intake resulted in an increase of 8 proteins with important immune functions in the blood plasma of lambs. Further proteomic studies will be necessary, particularly using the selected reaction monitoring approach, to describe in detail the role of specific colostrum proteins for immune transfer to the neonate.

AB - Colostrum intake is a key factor for newborn ruminant survival because the placenta does not allow the transfer of immune components. Therefore, newborn ruminants depend entirely on passive immunity transfer from the mother to the neonate, through the suckling of colostrum. Understanding the importance of specific colostrum proteins has gained significant attention in recent years. However, proteomics studies of sheep colostrum and their uptake in neonate lambs has not yet been presented. The aim of this study was to describe the proteomes of sheep colostrum and lamb blood plasma, using sodium dodecyl sulfate-PAGE for protein separation and in-gel digestion, followed by liquid chromatography-tandem mass spectrometry of resulting tryptic peptides for protein identification. An isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomics approach was subsequently used to provide relative quantification of how neonatal plasma protein concentrations change as an effect of colostrum intake. The results of this study describe the presence of 70 proteins in the ovine colostrum proteome. Furthermore, colostrum intake resulted in an increase of 8 proteins with important immune functions in the blood plasma of lambs. Further proteomic studies will be necessary, particularly using the selected reaction monitoring approach, to describe in detail the role of specific colostrum proteins for immune transfer to the neonate.

KW - colostrum

KW - immunity

KW - proteomics

KW - isobaric tag for relative and absolute quantitation (iTRAQ)

KW - PEPTIDOGLYCAN RECOGNITION PROTEINS

KW - INNATE IMMUNE-RESPONSE

KW - LOW-ABUNDANCE PROTEINS

KW - BOVINE MAMMARY-GLAND

KW - MAJORERA GOAT KIDS

KW - IMMUNOGLOBULIN-G

KW - A-IV

KW - PASSIVE TRANSFER

KW - SHORT-COMMUNICATION

KW - TRYPSIN-INHIBITOR

U2 - 10.3168/jds.2014-8143

DO - 10.3168/jds.2014-8143

M3 - Journal article

C2 - 25465637

VL - 98

SP - 135

EP - 147

JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

IS - 1

ER -