Cold acclimation preserves hindgut reabsorption capacity at low temperature in a chill-susceptible insect, Locusta migratoria

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Cold acclimation preserves hindgut reabsorption capacity at low temperature in a chill-susceptible insect, Locusta migratoria. / Gerber, Lucie; Kresse, Jean Claude; Šimek, Petr; Berková, Petra; Overgaard, Johannes.

I: Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, Bind 252, 110850, 02.2021.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Gerber, L, Kresse, JC, Šimek, P, Berková, P & Overgaard, J 2021, 'Cold acclimation preserves hindgut reabsorption capacity at low temperature in a chill-susceptible insect, Locusta migratoria', Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, bind 252, 110850. https://doi.org/10.1016/j.cbpa.2020.110850

APA

Gerber, L., Kresse, J. C., Šimek, P., Berková, P., & Overgaard, J. (2021). Cold acclimation preserves hindgut reabsorption capacity at low temperature in a chill-susceptible insect, Locusta migratoria. Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, 252, [110850]. https://doi.org/10.1016/j.cbpa.2020.110850

CBE

Gerber L, Kresse JC, Šimek P, Berková P, Overgaard J. 2021. Cold acclimation preserves hindgut reabsorption capacity at low temperature in a chill-susceptible insect, Locusta migratoria. Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology. 252:Article 110850. https://doi.org/10.1016/j.cbpa.2020.110850

MLA

Gerber, Lucie o.a.. "Cold acclimation preserves hindgut reabsorption capacity at low temperature in a chill-susceptible insect, Locusta migratoria". Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology. 2021. 252. https://doi.org/10.1016/j.cbpa.2020.110850

Vancouver

Gerber L, Kresse JC, Šimek P, Berková P, Overgaard J. Cold acclimation preserves hindgut reabsorption capacity at low temperature in a chill-susceptible insect, Locusta migratoria. Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology. 2021 feb;252. 110850. https://doi.org/10.1016/j.cbpa.2020.110850

Author

Gerber, Lucie ; Kresse, Jean Claude ; Šimek, Petr ; Berková, Petra ; Overgaard, Johannes. / Cold acclimation preserves hindgut reabsorption capacity at low temperature in a chill-susceptible insect, Locusta migratoria. I: Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology. 2021 ; Bind 252.

Bibtex

@article{b417906d71ba496da0e402ed3b9a5d49,
title = "Cold acclimation preserves hindgut reabsorption capacity at low temperature in a chill-susceptible insect, Locusta migratoria",
abstract = "Cold acclimation increases cold tolerance of chill-susceptible insects and the acclimation response often involves improved organismal ion balance and osmoregulatory function at low temperature. However, the physiological mechanisms underlying plasticity of ion regulatory capacity are largely unresolved. Here we used Ussing chambers to explore the effects of cold exposure on hindgut KCl reabsorption in cold- (11 °C) and warm-acclimated (30 °C) Locusta migratoria. Cooling (from 30 to 10 °C) reduced active reabsorption across recta from warm-acclimated locusts, while recta from cold-acclimated locusts maintained reabsorption at 10 °C. The differences in transport capacity were not linked to major rearrangements of membrane phospholipid profiles. Yet, the stimulatory effect of two signal transduction pathways were altered by temperature and/or acclimation. cAMP-stimulation increased reabsorption in both acclimation groups, with a strong stimulatory effect at 30 °C and a moderate stimulatory effect at 10 °C. cGMP-stimulation also increased reabsorption in both acclimation groups at 30 °C, but their response to cGMP differed at 10 °C. Recta from warm-acclimated locusts, characterised by reduced reabsorption at 10 °C, recovered reabsorption capacity following cGMP-stimulation at 10 °C. In contrast, recta from cold-acclimated locusts, characterised by sustained reabsorption at 10 °C, were unaffected by cGMP-stimulation. Furthermore, cold-exposed recta from warm-acclimated locusts were insensitive to bafilomycin-α1, a V-type H+-ATPase inhibitor, whereas this blocker reduced reabsorption across cold-exposed recta from cold-acclimated animals. In conclusion, bafilomycin-sensitive and cGMP-dependent transport mechanism(s) are likely blocked during cold exposure in warm-acclimated animals while preserved in cold-acclimated animals. These may in part explain the large differences in rectal ion transport capacity between acclimation groups at low temperature.",
keywords = "cGMP/cAMP signaling pathways, Cold tolerance, Membrane phospholipid, Temperature, Ussing chamber, V-type H-ATPase",
author = "Lucie Gerber and Kresse, {Jean Claude} and Petr {\v S}imek and Petra Berkov{\'a} and Johannes Overgaard",
note = "Funding Information: This work was supported by a grant from the Danish Council of Independent Research to J.O. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2021",
month = feb,
doi = "10.1016/j.cbpa.2020.110850",
language = "English",
volume = "252",
journal = "Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology",
issn = "1095-6433",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Cold acclimation preserves hindgut reabsorption capacity at low temperature in a chill-susceptible insect, Locusta migratoria

AU - Gerber, Lucie

AU - Kresse, Jean Claude

AU - Šimek, Petr

AU - Berková, Petra

AU - Overgaard, Johannes

N1 - Funding Information: This work was supported by a grant from the Danish Council of Independent Research to J.O. Publisher Copyright: © 2020 Elsevier Inc. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2021/2

Y1 - 2021/2

N2 - Cold acclimation increases cold tolerance of chill-susceptible insects and the acclimation response often involves improved organismal ion balance and osmoregulatory function at low temperature. However, the physiological mechanisms underlying plasticity of ion regulatory capacity are largely unresolved. Here we used Ussing chambers to explore the effects of cold exposure on hindgut KCl reabsorption in cold- (11 °C) and warm-acclimated (30 °C) Locusta migratoria. Cooling (from 30 to 10 °C) reduced active reabsorption across recta from warm-acclimated locusts, while recta from cold-acclimated locusts maintained reabsorption at 10 °C. The differences in transport capacity were not linked to major rearrangements of membrane phospholipid profiles. Yet, the stimulatory effect of two signal transduction pathways were altered by temperature and/or acclimation. cAMP-stimulation increased reabsorption in both acclimation groups, with a strong stimulatory effect at 30 °C and a moderate stimulatory effect at 10 °C. cGMP-stimulation also increased reabsorption in both acclimation groups at 30 °C, but their response to cGMP differed at 10 °C. Recta from warm-acclimated locusts, characterised by reduced reabsorption at 10 °C, recovered reabsorption capacity following cGMP-stimulation at 10 °C. In contrast, recta from cold-acclimated locusts, characterised by sustained reabsorption at 10 °C, were unaffected by cGMP-stimulation. Furthermore, cold-exposed recta from warm-acclimated locusts were insensitive to bafilomycin-α1, a V-type H+-ATPase inhibitor, whereas this blocker reduced reabsorption across cold-exposed recta from cold-acclimated animals. In conclusion, bafilomycin-sensitive and cGMP-dependent transport mechanism(s) are likely blocked during cold exposure in warm-acclimated animals while preserved in cold-acclimated animals. These may in part explain the large differences in rectal ion transport capacity between acclimation groups at low temperature.

AB - Cold acclimation increases cold tolerance of chill-susceptible insects and the acclimation response often involves improved organismal ion balance and osmoregulatory function at low temperature. However, the physiological mechanisms underlying plasticity of ion regulatory capacity are largely unresolved. Here we used Ussing chambers to explore the effects of cold exposure on hindgut KCl reabsorption in cold- (11 °C) and warm-acclimated (30 °C) Locusta migratoria. Cooling (from 30 to 10 °C) reduced active reabsorption across recta from warm-acclimated locusts, while recta from cold-acclimated locusts maintained reabsorption at 10 °C. The differences in transport capacity were not linked to major rearrangements of membrane phospholipid profiles. Yet, the stimulatory effect of two signal transduction pathways were altered by temperature and/or acclimation. cAMP-stimulation increased reabsorption in both acclimation groups, with a strong stimulatory effect at 30 °C and a moderate stimulatory effect at 10 °C. cGMP-stimulation also increased reabsorption in both acclimation groups at 30 °C, but their response to cGMP differed at 10 °C. Recta from warm-acclimated locusts, characterised by reduced reabsorption at 10 °C, recovered reabsorption capacity following cGMP-stimulation at 10 °C. In contrast, recta from cold-acclimated locusts, characterised by sustained reabsorption at 10 °C, were unaffected by cGMP-stimulation. Furthermore, cold-exposed recta from warm-acclimated locusts were insensitive to bafilomycin-α1, a V-type H+-ATPase inhibitor, whereas this blocker reduced reabsorption across cold-exposed recta from cold-acclimated animals. In conclusion, bafilomycin-sensitive and cGMP-dependent transport mechanism(s) are likely blocked during cold exposure in warm-acclimated animals while preserved in cold-acclimated animals. These may in part explain the large differences in rectal ion transport capacity between acclimation groups at low temperature.

KW - cGMP/cAMP signaling pathways

KW - Cold tolerance

KW - Membrane phospholipid

KW - Temperature

KW - Ussing chamber

KW - V-type H-ATPase

UR - http://www.scopus.com/inward/record.url?scp=85096964316&partnerID=8YFLogxK

U2 - 10.1016/j.cbpa.2020.110850

DO - 10.1016/j.cbpa.2020.110850

M3 - Journal article

C2 - 33221397

AN - SCOPUS:85096964316

VL - 252

JO - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

JF - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

SN - 1095-6433

M1 - 110850

ER -