Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation

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Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation. / Dahl, Marius A.; Areta, José Lisandro; Jeppesen, Per Bendix; Birk, Jesper Bratz; Johansen, Egil I.; Ingemann-Hansen, Thorsten; Hansen, Mette; Skålhegg, Bjørn Steen; Ivy, John L.; Wojtaszewski, Jørgen F.P.; Overgaard, Kristian; Jensen, Jørgen.

I: Journal of applied physiology (Bethesda, Md. : 1985), Bind 129, Nr. 2, 08.2020, s. 297-310.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Dahl, MA, Areta, JL, Jeppesen, PB, Birk, JB, Johansen, EI, Ingemann-Hansen, T, Hansen, M, Skålhegg, BS, Ivy, JL, Wojtaszewski, JFP, Overgaard, K & Jensen, J 2020, 'Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation', Journal of applied physiology (Bethesda, Md. : 1985), bind 129, nr. 2, s. 297-310. https://doi.org/10.1152/japplphysiol.00817.2019

APA

Dahl, M. A., Areta, J. L., Jeppesen, P. B., Birk, J. B., Johansen, E. I., Ingemann-Hansen, T., Hansen, M., Skålhegg, B. S., Ivy, J. L., Wojtaszewski, J. F. P., Overgaard, K., & Jensen, J. (2020). Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation. Journal of applied physiology (Bethesda, Md. : 1985), 129(2), 297-310. https://doi.org/10.1152/japplphysiol.00817.2019

CBE

Dahl MA, Areta JL, Jeppesen PB, Birk JB, Johansen EI, Ingemann-Hansen T, Hansen M, Skålhegg BS, Ivy JL, Wojtaszewski JFP, Overgaard K, Jensen J. 2020. Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation. Journal of applied physiology (Bethesda, Md. : 1985). 129(2):297-310. https://doi.org/10.1152/japplphysiol.00817.2019

MLA

Vancouver

Author

Dahl, Marius A. ; Areta, José Lisandro ; Jeppesen, Per Bendix ; Birk, Jesper Bratz ; Johansen, Egil I. ; Ingemann-Hansen, Thorsten ; Hansen, Mette ; Skålhegg, Bjørn Steen ; Ivy, John L. ; Wojtaszewski, Jørgen F.P. ; Overgaard, Kristian ; Jensen, Jørgen. / Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation. I: Journal of applied physiology (Bethesda, Md. : 1985). 2020 ; Bind 129, Nr. 2. s. 297-310.

Bibtex

@article{ecc117f7cd724208b364c1fe9e8b99eb,
title = "Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation",
abstract = "The present study compared the effects of postexercise carbohydrate plus protein (CHO+PROT) and carbohydrate (CHO)-only supplementation on muscle glycogen metabolism, anabolic cell signaling, and subsequent exercise performance. Nine endurance-trained males cycled twice to exhaustion (muscle glycogen decreased from ~495 to ~125 mmol/kg dry wt) and received either CHO only (1.2 g·kg-1·h-1) or CHO+PROT (0.8/0.4 g·kg-1·h-1) during the first 90 min of recovery. Glycogen content was similar before the performance test after 5 h of recovery. Glycogen synthase (GS) fractional activity increased after exhaustive exercise and remained activated 5 h after, despite substantial glycogen synthesis (176.1 ± 19.1 and 204.6 ± 27.0 mmol/kg dry wt in CHO and CHO+PROT, respectively; P = 0.15). Phosphorylation of GS at site 3 and site 2+2a remained low during recovery. After the 5-h recovery, cycling time to exhaustion was improved by CHO+PROT supplementation compared with CHO supplementation (54.6 ± 11.0 vs. 46.1 ± 9.8 min; P = 0.009). After the performance test, muscle glycogen was equally reduced in CHO+PROT and CHO. Akt Ser473 and p70s6k Thr389 phosphorylation was elevated after 5 h of recovery. There were no differences in Akt Ser473, p70s6k Thr389, or TSC2 Thr1462 phosphorylation between treatments. Nitrogen balance was positive in CHO+PROT (19.6 ± 7.6 mg nitrogen/kg; P = 0.04) and higher than CHO (-10.7 ± 6.3 mg nitrogen/kg; P = 0.009). CHO+PROT supplementation during exercise recovery improved subsequent endurance performance relative to consuming CHO only. This improved performance after CHO+PROT supplementation could not be accounted for by differences in glycogen metabolism or anabolic cell signaling, but may have been related to differences in nitrogen balance.NEW & NOTEWORTHY Endurance athletes competing consecutive days need optimal dietary intake during the recovery period. We report that coingestion of protein and carbohydrate soon after exhaustive exercise, compared with carbohydrate only, resulted in better performance the following day. The better performance after coingestion of protein and carbohydrate was not associated with a higher rate of glycogen synthesis or activation of anabolic signaling compared with carbohydrate only. Importantly, nitrogen balance was positive after coingestion of protein and carbohydrate, which was not the case after intake of carbohydrate only, suggesting that protein synthesis contributes to the better performance the following day.",
keywords = "Akt/PKB, exercise, glycogen synthase, nitrogen balance, protein synthesis",
author = "Dahl, {Marius A.} and Areta, {Jos{\'e} Lisandro} and Jeppesen, {Per Bendix} and Birk, {Jesper Bratz} and Johansen, {Egil I.} and Thorsten Ingemann-Hansen and Mette Hansen and Sk{\aa}lhegg, {Bj{\o}rn Steen} and Ivy, {John L.} and Wojtaszewski, {J{\o}rgen F.P.} and Kristian Overgaard and J{\o}rgen Jensen",
year = "2020",
month = aug,
doi = "10.1152/japplphysiol.00817.2019",
language = "English",
volume = "129",
pages = "297--310",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "AMER PHYSIOLOGICAL SOC",
number = "2",

}

RIS

TY - JOUR

T1 - Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation

AU - Dahl, Marius A.

AU - Areta, José Lisandro

AU - Jeppesen, Per Bendix

AU - Birk, Jesper Bratz

AU - Johansen, Egil I.

AU - Ingemann-Hansen, Thorsten

AU - Hansen, Mette

AU - Skålhegg, Bjørn Steen

AU - Ivy, John L.

AU - Wojtaszewski, Jørgen F.P.

AU - Overgaard, Kristian

AU - Jensen, Jørgen

PY - 2020/8

Y1 - 2020/8

N2 - The present study compared the effects of postexercise carbohydrate plus protein (CHO+PROT) and carbohydrate (CHO)-only supplementation on muscle glycogen metabolism, anabolic cell signaling, and subsequent exercise performance. Nine endurance-trained males cycled twice to exhaustion (muscle glycogen decreased from ~495 to ~125 mmol/kg dry wt) and received either CHO only (1.2 g·kg-1·h-1) or CHO+PROT (0.8/0.4 g·kg-1·h-1) during the first 90 min of recovery. Glycogen content was similar before the performance test after 5 h of recovery. Glycogen synthase (GS) fractional activity increased after exhaustive exercise and remained activated 5 h after, despite substantial glycogen synthesis (176.1 ± 19.1 and 204.6 ± 27.0 mmol/kg dry wt in CHO and CHO+PROT, respectively; P = 0.15). Phosphorylation of GS at site 3 and site 2+2a remained low during recovery. After the 5-h recovery, cycling time to exhaustion was improved by CHO+PROT supplementation compared with CHO supplementation (54.6 ± 11.0 vs. 46.1 ± 9.8 min; P = 0.009). After the performance test, muscle glycogen was equally reduced in CHO+PROT and CHO. Akt Ser473 and p70s6k Thr389 phosphorylation was elevated after 5 h of recovery. There were no differences in Akt Ser473, p70s6k Thr389, or TSC2 Thr1462 phosphorylation between treatments. Nitrogen balance was positive in CHO+PROT (19.6 ± 7.6 mg nitrogen/kg; P = 0.04) and higher than CHO (-10.7 ± 6.3 mg nitrogen/kg; P = 0.009). CHO+PROT supplementation during exercise recovery improved subsequent endurance performance relative to consuming CHO only. This improved performance after CHO+PROT supplementation could not be accounted for by differences in glycogen metabolism or anabolic cell signaling, but may have been related to differences in nitrogen balance.NEW & NOTEWORTHY Endurance athletes competing consecutive days need optimal dietary intake during the recovery period. We report that coingestion of protein and carbohydrate soon after exhaustive exercise, compared with carbohydrate only, resulted in better performance the following day. The better performance after coingestion of protein and carbohydrate was not associated with a higher rate of glycogen synthesis or activation of anabolic signaling compared with carbohydrate only. Importantly, nitrogen balance was positive after coingestion of protein and carbohydrate, which was not the case after intake of carbohydrate only, suggesting that protein synthesis contributes to the better performance the following day.

AB - The present study compared the effects of postexercise carbohydrate plus protein (CHO+PROT) and carbohydrate (CHO)-only supplementation on muscle glycogen metabolism, anabolic cell signaling, and subsequent exercise performance. Nine endurance-trained males cycled twice to exhaustion (muscle glycogen decreased from ~495 to ~125 mmol/kg dry wt) and received either CHO only (1.2 g·kg-1·h-1) or CHO+PROT (0.8/0.4 g·kg-1·h-1) during the first 90 min of recovery. Glycogen content was similar before the performance test after 5 h of recovery. Glycogen synthase (GS) fractional activity increased after exhaustive exercise and remained activated 5 h after, despite substantial glycogen synthesis (176.1 ± 19.1 and 204.6 ± 27.0 mmol/kg dry wt in CHO and CHO+PROT, respectively; P = 0.15). Phosphorylation of GS at site 3 and site 2+2a remained low during recovery. After the 5-h recovery, cycling time to exhaustion was improved by CHO+PROT supplementation compared with CHO supplementation (54.6 ± 11.0 vs. 46.1 ± 9.8 min; P = 0.009). After the performance test, muscle glycogen was equally reduced in CHO+PROT and CHO. Akt Ser473 and p70s6k Thr389 phosphorylation was elevated after 5 h of recovery. There were no differences in Akt Ser473, p70s6k Thr389, or TSC2 Thr1462 phosphorylation between treatments. Nitrogen balance was positive in CHO+PROT (19.6 ± 7.6 mg nitrogen/kg; P = 0.04) and higher than CHO (-10.7 ± 6.3 mg nitrogen/kg; P = 0.009). CHO+PROT supplementation during exercise recovery improved subsequent endurance performance relative to consuming CHO only. This improved performance after CHO+PROT supplementation could not be accounted for by differences in glycogen metabolism or anabolic cell signaling, but may have been related to differences in nitrogen balance.NEW & NOTEWORTHY Endurance athletes competing consecutive days need optimal dietary intake during the recovery period. We report that coingestion of protein and carbohydrate soon after exhaustive exercise, compared with carbohydrate only, resulted in better performance the following day. The better performance after coingestion of protein and carbohydrate was not associated with a higher rate of glycogen synthesis or activation of anabolic signaling compared with carbohydrate only. Importantly, nitrogen balance was positive after coingestion of protein and carbohydrate, which was not the case after intake of carbohydrate only, suggesting that protein synthesis contributes to the better performance the following day.

KW - Akt/PKB

KW - exercise

KW - glycogen synthase

KW - nitrogen balance

KW - protein synthesis

UR - http://www.scopus.com/inward/record.url?scp=85089358696&partnerID=8YFLogxK

U2 - 10.1152/japplphysiol.00817.2019

DO - 10.1152/japplphysiol.00817.2019

M3 - Journal article

C2 - 32584664

AN - SCOPUS:85089358696

VL - 129

SP - 297

EP - 310

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 2

ER -