TY - JOUR
T1 - Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation
AU - Dahl, Marius A.
AU - Areta, José Lisandro
AU - Jeppesen, Per Bendix
AU - Birk, Jesper Bratz
AU - Johansen, Egil I.
AU - Ingemann-Hansen, Thorsten
AU - Hansen, Mette
AU - Skålhegg, Bjørn Steen
AU - Ivy, John L.
AU - Wojtaszewski, Jørgen F.P.
AU - Overgaard, Kristian
AU - Jensen, Jørgen
PY - 2020/8
Y1 - 2020/8
N2 - Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation. J Appl Physiol 129: 297–310, 2020. First published June 25, 2020; doi:10.1152/japplphysiol.00817. 2019.—The present study compared the effects of postexercise carbohydrate plus protein (CHO+PROT) and carbohydrate (CHO)-only supplementation on muscle glycogen metabolism, anabolic cell signaling, and subsequent exercise performance. Nine endurance-trained males cycled twice to exhaustion (muscle glycogen decreased from ~495 to ~125 mmol/kg dry wt) and received either CHO only (1.2 g·kg
¯1·h¯
1) or CHO+PROT (0.8/0.4 g·kg
¯1·h¯
1) during the first 90 min of recovery. Glycogen content was similar before the performance test after 5 h of recovery. Glycogen synthase (GS) fractional activity increased after exhaustive exercise and remained activated 5 h after, despite substantial glycogen synthesis (176.1 ± 19.1 and 204.6 ± 27.0 mmol/kg dry wt in CHO and CHO+PROT, respectively; P = 0.15). Phosphorylation of GS at site 3 and site 2+2a remained low during recovery. After the 5-h recovery, cycling time to exhaustion was improved by CHO+PROT supplementation compared with CHO supplementation (54.6 ± 11.0 vs. 46.1 ± 9.8 min; P = 0.009). After the performance test, muscle glycogen was equally reduced in CHO+PROT and CHO. Akt Ser
473 and p70s6k Thr
389 phosphorylation was elevated after 5 h of recovery. There were no differences in Akt Ser
473, p70s6k Thr
389, or TSC2 Thr
1462 phosphorylation between treatments. Nitrogen balance was positive in CHO+PROT (19.6 ± 7.6 mg nitrogen/kg; P = 0.04) and higher than CHO (¯10.7 ± 6.3 mg nitrogen/kg; P = 0.009). CHO+PROT supplementation during exercise recovery improved subsequent endurance performance relative to consuming CHO only. This improved performance after CHO+PROT supplementation could not be accounted for by differences in glycogen metabolism or anabolic cell signaling, but may have been related to differences in nitrogen balance. report that coingestion of protein and carbohydrate soon after exhaustive exercise, compared with carbohydrate only, resulted in better performance the following day. The better performance after coingestion of protein and carbohydrate was not associated with a higher rate of glycogen synthesis or activation of anabolic signaling compared with carbohydrate only. Importantly, nitrogen balance was positive after coingestion of protein and carbohydrate, which was not the case after intake of carbohydrate only, suggesting that protein synthesis contributes to the better performance the following day.
AB - Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation. J Appl Physiol 129: 297–310, 2020. First published June 25, 2020; doi:10.1152/japplphysiol.00817. 2019.—The present study compared the effects of postexercise carbohydrate plus protein (CHO+PROT) and carbohydrate (CHO)-only supplementation on muscle glycogen metabolism, anabolic cell signaling, and subsequent exercise performance. Nine endurance-trained males cycled twice to exhaustion (muscle glycogen decreased from ~495 to ~125 mmol/kg dry wt) and received either CHO only (1.2 g·kg
¯1·h¯
1) or CHO+PROT (0.8/0.4 g·kg
¯1·h¯
1) during the first 90 min of recovery. Glycogen content was similar before the performance test after 5 h of recovery. Glycogen synthase (GS) fractional activity increased after exhaustive exercise and remained activated 5 h after, despite substantial glycogen synthesis (176.1 ± 19.1 and 204.6 ± 27.0 mmol/kg dry wt in CHO and CHO+PROT, respectively; P = 0.15). Phosphorylation of GS at site 3 and site 2+2a remained low during recovery. After the 5-h recovery, cycling time to exhaustion was improved by CHO+PROT supplementation compared with CHO supplementation (54.6 ± 11.0 vs. 46.1 ± 9.8 min; P = 0.009). After the performance test, muscle glycogen was equally reduced in CHO+PROT and CHO. Akt Ser
473 and p70s6k Thr
389 phosphorylation was elevated after 5 h of recovery. There were no differences in Akt Ser
473, p70s6k Thr
389, or TSC2 Thr
1462 phosphorylation between treatments. Nitrogen balance was positive in CHO+PROT (19.6 ± 7.6 mg nitrogen/kg; P = 0.04) and higher than CHO (¯10.7 ± 6.3 mg nitrogen/kg; P = 0.009). CHO+PROT supplementation during exercise recovery improved subsequent endurance performance relative to consuming CHO only. This improved performance after CHO+PROT supplementation could not be accounted for by differences in glycogen metabolism or anabolic cell signaling, but may have been related to differences in nitrogen balance. report that coingestion of protein and carbohydrate soon after exhaustive exercise, compared with carbohydrate only, resulted in better performance the following day. The better performance after coingestion of protein and carbohydrate was not associated with a higher rate of glycogen synthesis or activation of anabolic signaling compared with carbohydrate only. Importantly, nitrogen balance was positive after coingestion of protein and carbohydrate, which was not the case after intake of carbohydrate only, suggesting that protein synthesis contributes to the better performance the following day.
KW - Akt/PKB
KW - exercise
KW - glycogen synthase
KW - nitrogen balance
KW - protein synthesis
UR - http://www.scopus.com/inward/record.url?scp=85089358696&partnerID=8YFLogxK
U2 - 10.1152/japplphysiol.00817.2019
DO - 10.1152/japplphysiol.00817.2019
M3 - Journal article
C2 - 32584664
AN - SCOPUS:85089358696
SN - 8750-7587
VL - 129
SP - 297
EP - 310
JO - Journal of applied physiology (Bethesda, Md. : 1985)
JF - Journal of applied physiology (Bethesda, Md. : 1985)
IS - 2
ER -