TY - JOUR
T1 - Co-immobilization of Ce6 Sono/photosensitizer and Protonated Graphitic-Carbon Nitride on PCL/gelation Fibrous Scaffolds For Combined Sono-photodynamic Cancer Therapy
AU - Sun, Di
AU - Zhang, Zhongyang
AU - Chen, Mengya
AU - Zhang, Yanping
AU - Amagat, Jordi
AU - Kang, Shifei
AU - Zheng, Yuanyi
AU - Hu, Bing
AU - Chen, Menglin
PY - 2020
Y1 - 2020
N2 - Aiming at developing a moderate and efficient sono-photodynamic therapy for breast cancer, tissue engineering scaffolds may provide an easy and efficient strategy to eliminate serious side effects in conventional surgery or chemotherapy, and thus, they are highly desired. However, the development of ideal sono-photodynamic therapeutic scaffolds is always hindered by the poor stability and incompatibility between the different biomaterial components. Herein, the Food and Drug Administration (FDA)-approved sono/photosensitizer Chlorin e6 (Ce6) was successfully and tightly incorporated into electrospun polycaprolactone/gelatin (PG) scaffolds via positively charged protonated g-C3N4 nanosheets (pCN). The PG fibers were precoated with graphene oxide (GO) to enable the assembly of pCN on the surface through electrostatic interactions. The Ce6@pCN-GO-PG composite scaffolds exhibited good cytocompatibility and excellent sono-photodynamic activity, leading to distinctly boosted reactive oxygen species (ROS) generation and a 95.8% inactivation rate of breast cancer cells through a synergistic sono-photodynamic process triggered by an 808 nm laser and 1 MHz ultrasound (US) excitation, within the clinical therapeutic dose. The as-developed scaffolds with unique ultrasound cavitation therapeutic effects can be used not only for complete eradication of tumor cells after surgery but also as a cell behavior observation platform of sono-photodynamic cancer therapy.
AB - Aiming at developing a moderate and efficient sono-photodynamic therapy for breast cancer, tissue engineering scaffolds may provide an easy and efficient strategy to eliminate serious side effects in conventional surgery or chemotherapy, and thus, they are highly desired. However, the development of ideal sono-photodynamic therapeutic scaffolds is always hindered by the poor stability and incompatibility between the different biomaterial components. Herein, the Food and Drug Administration (FDA)-approved sono/photosensitizer Chlorin e6 (Ce6) was successfully and tightly incorporated into electrospun polycaprolactone/gelatin (PG) scaffolds via positively charged protonated g-C3N4 nanosheets (pCN). The PG fibers were precoated with graphene oxide (GO) to enable the assembly of pCN on the surface through electrostatic interactions. The Ce6@pCN-GO-PG composite scaffolds exhibited good cytocompatibility and excellent sono-photodynamic activity, leading to distinctly boosted reactive oxygen species (ROS) generation and a 95.8% inactivation rate of breast cancer cells through a synergistic sono-photodynamic process triggered by an 808 nm laser and 1 MHz ultrasound (US) excitation, within the clinical therapeutic dose. The as-developed scaffolds with unique ultrasound cavitation therapeutic effects can be used not only for complete eradication of tumor cells after surgery but also as a cell behavior observation platform of sono-photodynamic cancer therapy.
KW - Ce6 sono/photosensitizer
KW - protonated carbon nitride
KW - reactive oxygen species
KW - sono-photodynamic cancer therapy
KW - tissue engineering scaffolds
UR - http://www.scopus.com/inward/record.url?scp=85090869809&partnerID=8YFLogxK
U2 - 10.1021/acsami.0c08446
DO - 10.1021/acsami.0c08446
M3 - Journal article
C2 - 32794726
SN - 1944-8244
VL - 12
SP - 40728
EP - 40739
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
IS - 36
ER -