Circularization of the HIV-1 genome facilitates strand transfer during reverse transcription

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Circularization of the HIV-1 genome facilitates strand transfer during reverse transcription. / Beerens, Nancy; Kjems, Jørgen.

I: RNA, Bind 16, Nr. 6, 01.06.2010, s. 1226-35.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{129ca7f92db146e094842e1bfad9b894,
title = "Circularization of the HIV-1 genome facilitates strand transfer during reverse transcription",
abstract = "Two obligatory DNA strand transfers take place during reverse transcription of a retroviral RNA genome. The first strand transfer involves a jump from the 5' to the 3' terminal repeat (R) region positioned at each end of the viral genome. The process depends on base pairing between the cDNA synthesized from the 5' R region and the 3' R RNA. The tertiary conformation of the viral RNA genome may facilitate strand transfer by juxtaposing the 5' R and 3' R sequences that are 9 kb apart in the linear sequence. In this study, RNA sequences involved in an interaction between the 5' and 3' ends of the HIV-1 genome were mapped by mutational analysis. This interaction appears to be mediated mainly by a sequence in the extreme 3' end of the viral genome and in the gag open reading frame. Mutation of 3' R sequences was found to inhibit the 5'-3' interaction, which could be restored by a complementary mutation in the 5' gag region. Furthermore, we find that circularization of the HIV-1 genome does not affect the initiation of reverse transcription, but stimulates the first strand transfer during reverse transcription in vitro, underscoring the functional importance of the interaction.",
keywords = "3' Untranslated Regions, 5' Untranslated Regions, Base Sequence, Gene Transfer Techniques, Genome, Viral, HIV-1, Molecular Sequence Data, Mutagenesis, Nucleic Acid Conformation, RNA, RNA, Viral, RNA-Directed DNA Polymerase, Retroelements, Transcription, Genetic, Virus Replication",
author = "Nancy Beerens and J{\o}rgen Kjems",
year = "2010",
month = "6",
day = "1",
doi = "10.1261/rna.2039610",
language = "English",
volume = "16",
pages = "1226--35",
journal = "RNA",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
number = "6",

}

RIS

TY - JOUR

T1 - Circularization of the HIV-1 genome facilitates strand transfer during reverse transcription

AU - Beerens, Nancy

AU - Kjems, Jørgen

PY - 2010/6/1

Y1 - 2010/6/1

N2 - Two obligatory DNA strand transfers take place during reverse transcription of a retroviral RNA genome. The first strand transfer involves a jump from the 5' to the 3' terminal repeat (R) region positioned at each end of the viral genome. The process depends on base pairing between the cDNA synthesized from the 5' R region and the 3' R RNA. The tertiary conformation of the viral RNA genome may facilitate strand transfer by juxtaposing the 5' R and 3' R sequences that are 9 kb apart in the linear sequence. In this study, RNA sequences involved in an interaction between the 5' and 3' ends of the HIV-1 genome were mapped by mutational analysis. This interaction appears to be mediated mainly by a sequence in the extreme 3' end of the viral genome and in the gag open reading frame. Mutation of 3' R sequences was found to inhibit the 5'-3' interaction, which could be restored by a complementary mutation in the 5' gag region. Furthermore, we find that circularization of the HIV-1 genome does not affect the initiation of reverse transcription, but stimulates the first strand transfer during reverse transcription in vitro, underscoring the functional importance of the interaction.

AB - Two obligatory DNA strand transfers take place during reverse transcription of a retroviral RNA genome. The first strand transfer involves a jump from the 5' to the 3' terminal repeat (R) region positioned at each end of the viral genome. The process depends on base pairing between the cDNA synthesized from the 5' R region and the 3' R RNA. The tertiary conformation of the viral RNA genome may facilitate strand transfer by juxtaposing the 5' R and 3' R sequences that are 9 kb apart in the linear sequence. In this study, RNA sequences involved in an interaction between the 5' and 3' ends of the HIV-1 genome were mapped by mutational analysis. This interaction appears to be mediated mainly by a sequence in the extreme 3' end of the viral genome and in the gag open reading frame. Mutation of 3' R sequences was found to inhibit the 5'-3' interaction, which could be restored by a complementary mutation in the 5' gag region. Furthermore, we find that circularization of the HIV-1 genome does not affect the initiation of reverse transcription, but stimulates the first strand transfer during reverse transcription in vitro, underscoring the functional importance of the interaction.

KW - 3' Untranslated Regions

KW - 5' Untranslated Regions

KW - Base Sequence

KW - Gene Transfer Techniques

KW - Genome, Viral

KW - HIV-1

KW - Molecular Sequence Data

KW - Mutagenesis

KW - Nucleic Acid Conformation

KW - RNA

KW - RNA, Viral

KW - RNA-Directed DNA Polymerase

KW - Retroelements

KW - Transcription, Genetic

KW - Virus Replication

U2 - 10.1261/rna.2039610

DO - 10.1261/rna.2039610

M3 - Journal article

C2 - 20430859

VL - 16

SP - 1226

EP - 1235

JO - RNA

JF - RNA

SN - 1355-8382

IS - 6

ER -