Chitosan/siRNA nanoparticles encapsulated in PLGA nanofibers for siRNA delivery

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Chitosan/siRNA nanoparticles encapsulated in PLGA nanofibers for siRNA delivery. / Chen, Menglin; Gao, Shan; Dong, Mingdong; Song, Jie; Yang, Chuanxu; Howard, Kenneth Alan ; Kjems, Jørgen ; Besenbacher, Flemming.

I: A C S Nano, Bind 6, Nr. 6, 26.06.2012, s. 4835-4844.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Bibtex

@article{f02453f460cf49d094f52decddf33b1f,

title = "Chitosan/siRNA nanoparticles encapsulated in PLGA nanofibers for siRNA delivery",

abstract = "Composite nanofibers of biodegradable poly(d,l-lactic-co-glycolic acid) (PLGA) encapsulating chitosan/siRNA nanoparticles (NPs) were prepared by electrospinning. Acidic/alkaline hydrolysis and a bulk/surface degradation mechanism were investigated in order to achieve an optimized release profile for prolonged and efficient gene silencing. Thermo-controlled AFM in situ imaging not only revealed the integrity of the encapsulated chitosan/siRNA polyplex but also shed light on the decreasing Tg of PLGA on the fiber surfaces during release. A triphasic release profile based on bulk erosion was obtained at pH 7.4, while a triphasic release profile involving both surface erosion and bulk erosion was obtained at pH 5.5. A short alkaline pretreatment provided a homogeneous hydrolysis and consequently a nearly zero-order release profile. The interesting release profile was further investigated for siRNA transfection, where the encapsulated chitosan/siRNA NPs exhibited up to 50% EGFP gene silencing activity after 48 h post-transfection on H1299 cells.",

keywords = "nanofibers, chitosan, siRNA, electrospinning, release, gene silencing",

author = "Menglin Chen and Shan Gao and Mingdong Dong and Jie Song and Chuanxu Yang and Howard, {Kenneth Alan} and J{\o}rgen Kjems and Flemming Besenbacher",

year = "2012",

month = jun,

day = "26",

doi = "10.1021/nn300106t",

language = "English",

volume = "6",

pages = "4835--4844",

journal = "A C S Nano",

issn = "1936-0851",

publisher = "American Chemical Society",

number = "6",

}

RIS

TY - JOUR

T1 - Chitosan/siRNA nanoparticles encapsulated in PLGA nanofibers for siRNA delivery

AU - Chen, Menglin

AU - Gao, Shan

AU - Dong, Mingdong

AU - Song, Jie

AU - Yang, Chuanxu

AU - Howard, Kenneth Alan

AU - Kjems, Jørgen

AU - Besenbacher, Flemming

PY - 2012/6/26

Y1 - 2012/6/26

N2 - Composite nanofibers of biodegradable poly(d,l-lactic-co-glycolic acid) (PLGA) encapsulating chitosan/siRNA nanoparticles (NPs) were prepared by electrospinning. Acidic/alkaline hydrolysis and a bulk/surface degradation mechanism were investigated in order to achieve an optimized release profile for prolonged and efficient gene silencing. Thermo-controlled AFM in situ imaging not only revealed the integrity of the encapsulated chitosan/siRNA polyplex but also shed light on the decreasing Tg of PLGA on the fiber surfaces during release. A triphasic release profile based on bulk erosion was obtained at pH 7.4, while a triphasic release profile involving both surface erosion and bulk erosion was obtained at pH 5.5. A short alkaline pretreatment provided a homogeneous hydrolysis and consequently a nearly zero-order release profile. The interesting release profile was further investigated for siRNA transfection, where the encapsulated chitosan/siRNA NPs exhibited up to 50% EGFP gene silencing activity after 48 h post-transfection on H1299 cells.

AB - Composite nanofibers of biodegradable poly(d,l-lactic-co-glycolic acid) (PLGA) encapsulating chitosan/siRNA nanoparticles (NPs) were prepared by electrospinning. Acidic/alkaline hydrolysis and a bulk/surface degradation mechanism were investigated in order to achieve an optimized release profile for prolonged and efficient gene silencing. Thermo-controlled AFM in situ imaging not only revealed the integrity of the encapsulated chitosan/siRNA polyplex but also shed light on the decreasing Tg of PLGA on the fiber surfaces during release. A triphasic release profile based on bulk erosion was obtained at pH 7.4, while a triphasic release profile involving both surface erosion and bulk erosion was obtained at pH 5.5. A short alkaline pretreatment provided a homogeneous hydrolysis and consequently a nearly zero-order release profile. The interesting release profile was further investigated for siRNA transfection, where the encapsulated chitosan/siRNA NPs exhibited up to 50% EGFP gene silencing activity after 48 h post-transfection on H1299 cells.

KW - nanofibers

KW - chitosan

KW - siRNA

KW - electrospinning

KW - release

KW - gene silencing

UR - http://www.scopus.com/inward/record.url?scp=84862856412&partnerID=8YFLogxK

U2 - 10.1021/nn300106t

DO - 10.1021/nn300106t

M3 - Journal article

C2 - 22621383

AN - SCOPUS:84862856412

VL - 6

SP - 4835

EP - 4844

JO - A C S Nano

JF - A C S Nano

SN - 1936-0851

IS - 6

ER -