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Characterization of components released by alkali disruption of simian virus 40.

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Standard

Characterization of components released by alkali disruption of simian virus 40. / Christiansen, Gunna; Landers, T; Griffith, J; Berg, P.

I: J. Virol., Bind 21, 1977, s. 1079-1084.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Christiansen, G, Landers, T, Griffith, J & Berg, P 1977, 'Characterization of components released by alkali disruption of simian virus 40.', J. Virol., bind 21, s. 1079-1084.

APA

Christiansen, G., Landers, T., Griffith, J., & Berg, P. (1977). Characterization of components released by alkali disruption of simian virus 40. J. Virol., 21, 1079-1084.

CBE

Christiansen G, Landers T, Griffith J, Berg P. 1977. Characterization of components released by alkali disruption of simian virus 40. J. Virol. 21:1079-1084.

MLA

Vancouver

Author

Christiansen, Gunna ; Landers, T ; Griffith, J ; Berg, P. / Characterization of components released by alkali disruption of simian virus 40. I: J. Virol. 1977 ; Bind 21. s. 1079-1084.

Bibtex

@article{0e33199098fd11dcbee902004c4f4f50,
title = "Characterization of components released by alkali disruption of simian virus 40.",
abstract = "Treatment of simian virus 40 (SV40) particles at pH 9.8 in the presence of 1 mM dithiothreitol for 5 min at 37 degrees C disrupted the virions into a 60S DNA-protein complex and DNA-free 7S protein particles. The DNA-protein complex contained approximately equal amounts of DNA and protein, and appeared by electron microscopy to be relaxed circular structures with an average of 21 beads joined by short, thin bridges. The major protein components in the complex were host cell histones, but SV40-specific proteins VP3 and VP2 were also present. The 7S protein particles were almost exclusively VP1 and, in negatively stained samples, resembled the capsomer structures of intact virions.",
author = "Gunna Christiansen and T Landers and J Griffith and P Berg",
year = "1977",
language = "English",
volume = "21",
pages = "1079--1084",
journal = "J. Virol.",

}

RIS

TY - JOUR

T1 - Characterization of components released by alkali disruption of simian virus 40.

AU - Christiansen, Gunna

AU - Landers, T

AU - Griffith, J

AU - Berg, P

PY - 1977

Y1 - 1977

N2 - Treatment of simian virus 40 (SV40) particles at pH 9.8 in the presence of 1 mM dithiothreitol for 5 min at 37 degrees C disrupted the virions into a 60S DNA-protein complex and DNA-free 7S protein particles. The DNA-protein complex contained approximately equal amounts of DNA and protein, and appeared by electron microscopy to be relaxed circular structures with an average of 21 beads joined by short, thin bridges. The major protein components in the complex were host cell histones, but SV40-specific proteins VP3 and VP2 were also present. The 7S protein particles were almost exclusively VP1 and, in negatively stained samples, resembled the capsomer structures of intact virions.

AB - Treatment of simian virus 40 (SV40) particles at pH 9.8 in the presence of 1 mM dithiothreitol for 5 min at 37 degrees C disrupted the virions into a 60S DNA-protein complex and DNA-free 7S protein particles. The DNA-protein complex contained approximately equal amounts of DNA and protein, and appeared by electron microscopy to be relaxed circular structures with an average of 21 beads joined by short, thin bridges. The major protein components in the complex were host cell histones, but SV40-specific proteins VP3 and VP2 were also present. The 7S protein particles were almost exclusively VP1 and, in negatively stained samples, resembled the capsomer structures of intact virions.

M3 - Journal article

VL - 21

SP - 1079

EP - 1084

JO - J. Virol.

JF - J. Virol.

ER -