Aarhus University Seal / Aarhus Universitets segl

Characterization of circular RNA concatemers

Publikation: Bidrag til bog/antologi/rapport/proceedingBidrag til bog/antologiForskningpeer review

Circular RNAs (circRNAs) constitute a novel subset in the fascinating world of noncoding RNA, and they are found in practically all eukaryotes. Most of them exhibit low expression levels but some are extremely abundant. Typically, circRNAs are studied by RT-PCR-based assays, but for certain types of analyses this technique is not suitable. Circular RNA with repetitive exons (circular concatemers) has been observed by us and others when transiently expressing circRNAs in cells, however techniques and assays to study these species have not been established. Here, this chapter outlines three biochemical assays (RNase R-, RNase H-, and alkaline-treatment) that combined with northern blotting are useful to study circRNAs in general and circular RNA concatemers in particular.

TitelCircular RNAs : Methods and Protocols
RedaktørerC. Dietrerich, A. Papantonis
Antal sider15
UdgivelsesstedNew York
ForlagHumana Press
Udgivelsesår11 jan. 2018
ISBN (trykt)978-1-4939-7561-7
ISBN (Elektronisk)978-1-4939-7562-4
StatusUdgivet - 11 jan. 2018
SerietitelMethods in Molecular Biology

Se relationer på Aarhus Universitet Citationsformater

ID: 121059569