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Characterization of circular RNA concatemers

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Circular RNAs (circRNAs) constitute a novel subset in the fascinating world of noncoding RNA, and they are found in practically all eukaryotes. Most of them exhibit low expression levels but some are extremely abundant. Typically, circRNAs are studied by RT-PCR-based assays, but for certain types of analyses this technique is not suitable. Circular RNA with repetitive exons (circular concatemers) has been observed by us and others when transiently expressing circRNAs in cells, however techniques and assays to study these species have not been established. Here, this chapter outlines three biochemical assays (RNase R-, RNase H-, and alkaline-treatment) that combined with northern blotting are useful to study circRNAs in general and circular RNA concatemers in particular.

OriginalsprogEngelsk
TitelCircular RNAs : Methods and Protocols
RedaktørerC. Dietrerich, A. Papantonis
Antal sider15
Vol/bind1724
UdgivelsesstedNew York
ForlagHumana Press
Udgivelsesår11 jan. 2018
Sider143-157
ISBN (trykt)978-1-4939-7561-7
ISBN (Elektronisk)978-1-4939-7562-4
DOI
StatusUdgivet - 11 jan. 2018
SerietitelMethods in Molecular Biology
Vol/bind1724
ISSN1064-3745

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