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Characterisation and 3D structure of melanomacrophage centers in shorthorn sculpins (Myoxocephalus scorpius)

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Characterisation and 3D structure of melanomacrophage centers in shorthorn sculpins (Myoxocephalus scorpius). / Dang, Mai; Nowell, Cameron; Nguyen, Tam; Bach, Lis; Sonne, Christian; Nørregaard, Rasmus; Stride, Megan; Nowak, Barbara.

I: Tissue and Cell, Bind 57, 2019, s. 34-41.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Dang, Mai ; Nowell, Cameron ; Nguyen, Tam ; Bach, Lis ; Sonne, Christian ; Nørregaard, Rasmus ; Stride, Megan ; Nowak, Barbara. / Characterisation and 3D structure of melanomacrophage centers in shorthorn sculpins (Myoxocephalus scorpius). I: Tissue and Cell. 2019 ; Bind 57. s. 34-41.

Bibtex

@article{836e7cc4c45c42d8994435053d073b36,
title = "Characterisation and 3D structure of melanomacrophage centers in shorthorn sculpins (Myoxocephalus scorpius)",
abstract = "Melanomacrophage centres (MMCs) are distinct aggregations of pigment-containing cells in internal organs of fish, amphibians and reptiles. Although MMCs are commonly used as biomarkers for anthropogenic exposure in many environmental monitoring programs, a substantial knowledge on characteristics of MMCs is required prior to the assessment of MMC responses. The present study was the first to determine the 3D structure of splenic MMCs of a fish from a number of consecutive histology sections by use of the Fiji and AutoCad software. Most splenic MMCs of shorthorn sculpins (Myoxocephalus scorpius) had spherical shape and limited variation in size (maximum diameter). We confirmed the close relationship between MMCs and blood vessels in spleen of shorthorn sculpins as 97% of investigated MMCs (60 whole MMCs over 510 μm thickness of the samples) were closely associated with splenic blood capillaries (mainly ellipsoids) at least once in a set of consecutive sections. In this paper, we describe variations in morphology, density, size, area, distribution, pigments and response to pathogens of MMC populations from different organs (spleen, kidney, liver, pancreas and gills). Additionally, we provide evidence suggesting the presence and dominance of pheomelanin in MMCs of shorthorn sculpins.",
keywords = "3D structure, BIOMARKERS, BLUEFIN TUNA, HEAD KIDNEY, INDICATORS, LIVER, MELANO-MACROPHAGE CENTERS, Melanin, Melanomacrophage centers, NEOTROPICAL FISH, Pheomelanin, QUANTITATIVE-ANALYSIS, SEASONAL-VARIATION, SPLEEN, Shorthorn sculpin",
author = "Mai Dang and Cameron Nowell and Tam Nguyen and Lis Bach and Christian Sonne and Rasmus N{\o}rregaard and Megan Stride and Barbara Nowak",
year = "2019",
doi = "10.1016/j.tice.2019.02.003",
language = "English",
volume = "57",
pages = "34--41",
journal = "Tissue and Cell",
issn = "0040-8166",
publisher = "Churchill Livingstone",

}

RIS

TY - JOUR

T1 - Characterisation and 3D structure of melanomacrophage centers in shorthorn sculpins (Myoxocephalus scorpius)

AU - Dang, Mai

AU - Nowell, Cameron

AU - Nguyen, Tam

AU - Bach, Lis

AU - Sonne, Christian

AU - Nørregaard, Rasmus

AU - Stride, Megan

AU - Nowak, Barbara

PY - 2019

Y1 - 2019

N2 - Melanomacrophage centres (MMCs) are distinct aggregations of pigment-containing cells in internal organs of fish, amphibians and reptiles. Although MMCs are commonly used as biomarkers for anthropogenic exposure in many environmental monitoring programs, a substantial knowledge on characteristics of MMCs is required prior to the assessment of MMC responses. The present study was the first to determine the 3D structure of splenic MMCs of a fish from a number of consecutive histology sections by use of the Fiji and AutoCad software. Most splenic MMCs of shorthorn sculpins (Myoxocephalus scorpius) had spherical shape and limited variation in size (maximum diameter). We confirmed the close relationship between MMCs and blood vessels in spleen of shorthorn sculpins as 97% of investigated MMCs (60 whole MMCs over 510 μm thickness of the samples) were closely associated with splenic blood capillaries (mainly ellipsoids) at least once in a set of consecutive sections. In this paper, we describe variations in morphology, density, size, area, distribution, pigments and response to pathogens of MMC populations from different organs (spleen, kidney, liver, pancreas and gills). Additionally, we provide evidence suggesting the presence and dominance of pheomelanin in MMCs of shorthorn sculpins.

AB - Melanomacrophage centres (MMCs) are distinct aggregations of pigment-containing cells in internal organs of fish, amphibians and reptiles. Although MMCs are commonly used as biomarkers for anthropogenic exposure in many environmental monitoring programs, a substantial knowledge on characteristics of MMCs is required prior to the assessment of MMC responses. The present study was the first to determine the 3D structure of splenic MMCs of a fish from a number of consecutive histology sections by use of the Fiji and AutoCad software. Most splenic MMCs of shorthorn sculpins (Myoxocephalus scorpius) had spherical shape and limited variation in size (maximum diameter). We confirmed the close relationship between MMCs and blood vessels in spleen of shorthorn sculpins as 97% of investigated MMCs (60 whole MMCs over 510 μm thickness of the samples) were closely associated with splenic blood capillaries (mainly ellipsoids) at least once in a set of consecutive sections. In this paper, we describe variations in morphology, density, size, area, distribution, pigments and response to pathogens of MMC populations from different organs (spleen, kidney, liver, pancreas and gills). Additionally, we provide evidence suggesting the presence and dominance of pheomelanin in MMCs of shorthorn sculpins.

KW - 3D structure

KW - BIOMARKERS

KW - BLUEFIN TUNA

KW - HEAD KIDNEY

KW - INDICATORS

KW - LIVER

KW - MELANO-MACROPHAGE CENTERS

KW - Melanin

KW - Melanomacrophage centers

KW - NEOTROPICAL FISH

KW - Pheomelanin

KW - QUANTITATIVE-ANALYSIS

KW - SEASONAL-VARIATION

KW - SPLEEN

KW - Shorthorn sculpin

UR - http://www.scopus.com/inward/record.url?scp=85061395922&partnerID=8YFLogxK

U2 - 10.1016/j.tice.2019.02.003

DO - 10.1016/j.tice.2019.02.003

M3 - Journal article

C2 - 30947961

AN - SCOPUS:85061395922

VL - 57

SP - 34

EP - 41

JO - Tissue and Cell

JF - Tissue and Cell

SN - 0040-8166

ER -